Kunyera, Robert M. (2015) Comparative Study for the Analysis of the Microbiota of the Glans Penis and the Vagina of the Olive Baboons (Papio Anubis). Masters thesis, University of Nairobi.
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PDF (Comparative Study for the Analysis of the Microbiota of the Glans Penis and the Vagina of the Olive Baboons (Papio Anubis))
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Abstract
The human reproductive system is one of the important microbial habitats that harbor different species of bacteria in variable quantities and relative proportions that are known to have important effects on health, both normal and pathological effect. The baboon models have been useful to provide detailed understanding of the human reproduction, Reproductive physiology; fertility mechanism, immunology, pathology and anatomy in humans due to their phylogenetic similarities. However little is known about the composition of the vaginal and glans penis microbial ecosystem. The knowledge of the composition of vaginal microbial and the gland penis ecosystem is essential for understanding the etiology, prevention and treatment of urinary tract infection, sexually transmitted disease and prostatitis in males. The study was a laboratory experimental case-control study design carried out at the institute of primate research aimed at comparing the microbiota of the baboon vaginal and glans penis microbial flora. Blood, vaginal and urethral swabs samples were collected. Gram stained and cultures were done aerobically and anaerobically then scored using the Nugent criteria. Biochemical identification of the cultures was performed using the API® kits and analyzed by apiwebTM standalone identification software. The Total free serum prostate specific antigens levels in male baboon were determined using electrochemiluminescence immunoassay “ECLIA” (Roche Diagnostics). The study was approved by the institute of primate research Ethics and Institutional Review Committee. Data was analyzed using Microsoft excel 2010 version. In this study, the baboon (Papio Anubis) vaginal and lower male genital tract microbiota are heterogenous in terms of species composition, but the vaginal is typified by a scarcity of lactobacilli unlike the women, while the majority of the colonizing organism in both sex being staphylococci aureus more than 60% of all the animals. The most isolated and identified bacterial species was Lactobacilli, Staphylococci, streptococcus Clostridia, Bacilli, Corynebacterium, gram-negative rods mostly E.coli in females only, and other species of Gram-positive rods, cocci and Candida species with c. albicans the most predominant in females but absentee in males.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | S Agriculture > SF Animal culture |
| Divisions: | Africana |
| Depositing User: | Mr. Senay Abate |
| Date Deposited: | 01 Aug 2016 12:19 |
| Last Modified: | 20 Jul 2017 09:47 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/1112 |
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