Macro-and Micro-Propagation of Red Stinkwood [prunus africana (Hook. F.) Kalkman (1965)] in Kenya

Muchoki, Stephen Macharia (2000) Macro-and Micro-Propagation of Red Stinkwood [prunus africana (Hook. F.) Kalkman (1965)] in Kenya. Masters thesis, Kenyatta University.

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Abstract

Protocols for micro-propagation and macro-propagation of red stinkwood [Prunus africana (Hook F.) Kalkman (1965)] were developed. Shoot tip and uni-nodal explants were cultured in vitro on either full strength or modified Murashige and Skoog's (1962) medium augmented with plant growth regulating substances. The effects of different combinations and concentrations of 6 - benzylaminopurine (BAP), 6 - furfurylaminopurine (kinetin), 6 - (4-hydroxy-3-methyl-but-trans-2- enyl) aminopurine (zeatin), indole acetic acid (IAA), indole butyric acid (IBA) and a-naphthalene acetic acid (NAA) on axillary shoot proliferation were studied for P. africana shoot explants. The presence of a cytokinin either alone or in combination with an auxin was found to be necessary for the induction and proliferation of axillary shoots on explants. Media containing zeatin alone or in combination with an auxin gave the best results. The influence of different auxins on in vitro rooting of P. africana second generation microcuttings was also investigated. The addition of an exogenous auxin Was found to be necessary for root induction. Irrespective of the concentrations used, both IBA and NAA induced low rooting percentages <40 %). However, NAA was significantly better than IBA on inducing root in vitro on P. africana shoots. The effects of different combinations and concentration ofthe growth regulating substances 2, 4-dichlorophenoxy acetic acid (2,4-0), lAA. NAA, BAP and zeatin on callus induction and subsequent regeneration of shoot was also investigated. Media supplemented with 2,4-0 or 1\AA gave the fastest growing callus. However no regeneration of shoots from callus was observed. The possibility of regenerating P. africana from stem cuttings was also investigated. Stem cuttings derived from juvenile plants regenerated roots easily. However, cuttings collected from mature plants gave low rooting percentages <10 %). Auxins proved to be essential for the induction of roots on stem cuttings with IBA proving to be superior to both IAA and NAA in inducing rooting of P. africana stem cuttings. The results of this study indicate that it is possible to regenerate P. cfricana using both macro-propagation and micro-propagation. The use of the above two methods of vegetative propagation in conjunction with seed conjunction will help in alleviating the current biological problems that limit reforestation of P. africana and at the same time encourage investment through improved yields better quality of bark and shorter breeding cycles.

Item Type: Thesis (Masters)
Subjects: Q Science > QK Botany
S Agriculture > SD Forestry
Divisions: Africana
Depositing User: Tim Khabala
Date Deposited: 06 Nov 2017 11:47
Last Modified: 06 Nov 2017 11:47
URI: http://thesisbank.jhia.ac.ke/id/eprint/2633

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