Chronic Effects of the Aqueous Leaf Extract of Syzygium guineense on Some Blood Parameters and Histopathology of Liver and Kidney of Mice

Amare, Girmay (2009) Chronic Effects of the Aqueous Leaf Extract of Syzygium guineense on Some Blood Parameters and Histopathology of Liver and Kidney of Mice. Masters thesis, Addis Ababa University.

PDF (Chronic Effects of the Aqueous Leaf Extract of Syzygium guineense on Some Blood Parameters and Histopathology of Liver and Kidney of Mice) Amare, Girmay.pdf Restricted to Repository staff only Download (2MB) | Request a copy

Abstract

Syzygium guineense is a tropical evergreen tree commonly used by traditional medicine practitioners in Ethiopia for the treatment of diarrhea. In this investigation, the histopathologic effects of S. guineense leaf extract on the liver and kidney were examined and hematological examinations were also carried out in mice. Oral treatment of the aqueous extract was given for the albino mice weighing 25-30g, at doses of 200, 400 and 600mg/kg bw daily for six weeks and then sacrificed following the blood collection for hematological examination. Tissues were harvested and processed for light microscopic investigations and for the photomicrographic procedure. Similarly, another group of mice was given the LD50, to observe Key words: S. guineense, histopathology, hematology, liver, kidney. the histopathological effect of the extract. Light microscopic investigations in the liver and kidney tissue sections of the mice showed morphological changes at the highest doses of the extract. The liver revealed hemmorhegic centrilobular necrosis and cytoplasmic vacoulation of the hepatocytes: and in the kidney tubulointerstitial inflammation, shrinkage and congestion of the glomerulus were observed at higher doses. Hematological investigations showed lowered Hgb concentrations that might indicate the development of mild anemia. Decreased body weight gain and increased organ weights of the liver and kidney were observed in contrast to the control. The results of the study in mice may suggest that higher doses of the aqueous extract of S. guineense might have some constituents that alter the histology and hematological parameters. From these observations, it can be inferred that long term use of higher doses of S. guineense may be associated with tissue structural damage of the liver and kidneys.

Item Type:	Thesis (Masters)
Subjects:	Q Science > QM Human anatomy R Medicine > RB Pathology
Divisions:	Africana
Depositing User:	Tim Khabala
Date Deposited:	18 May 2018 14:31
Last Modified:	18 May 2018 14:31
URI:	http://thesisbank.jhia.ac.ke/id/eprint/4028

Actions (login required)

View Item