Abiy, Tewelde (2015) Evaluation and Optimization of Agro-industrial Wastes for Conidial Production of Metarhizium and Beauveria Isolates under Solid State Fermentation. Masters thesis, Addis Ababa University.
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Abstract
Two isolates of Beauveria and three isolates of Metarhizium were grown on different agricultural wastes to evaluate their conidia production potential under SSF system. Coffee husk, Tea waste, Wheat bran and Vegetable wastes were used as substrates to determine maximum conidiation of the isolates. Among these vegetable wastes were the best medium to yield 5.77±1.53 (107), 5.80±0.72 (107), 4.44 ± 0.55(107) and 5.58 ± 0.66 (107) conidia/gram of substrate under quantitative assessment for isolates (AUBI2, AUMI1, AUMI2 and AUMI3 respectively) at 60% moisture content. The highest conidia count record of AUBI1 6.34 ± 2.27 (107) conidia/gram was obtained at 60% moisture content on wheat bran. It has been observed that two sample t-Test statistical analyses of conidial yield of vegetable wastes was significantly different from 2mm and 4mm particle size of coffee husk used as substrates. The optimization for temperature indicated that all substrates supported maximum conidia yield with-in the range of temperature from 27 to 300C. Beauveria isolate AUBI1 was produced 11.63 ± 8.34 (107) conidia/gram of substrate at coffee husk when treated at 3.5 pH value. The pH values used in the present study for optimization best favored only for coffee husk as substrate. The high conidia yielding substrates were best productive at their own natural at 6.29 for Vegetable wastes, 6.63 for Wheat bran and 5.4 for Tea waste pH values. Vegetable wastes supported AUBI1, AUBI2, AUMI1, AUMI2 and AUMI3 to produce high conidia yield of 4.31 ± 1.18 (107), 5.07 ± 0.76 (107), 3.43 ± 0.30 (107), 1.86 ± 0.16 (107) and 5.17 ± 0.61 (107) conidia/gram of substrate at optimum incubation period of 21 days. The optimum incubation period of the fungal isolates on wheat bran is 3 - 4 weeks under sufficient exposure to light. AUBI1, AUBI2 and AUMI1 produce high conidia under exposure to light but AUMI2 and AUMI3 at dark condition when cultivated on vegetable wastes. Therefore, as successful microbial control of insect pests depends on cheap and large scale industrial productivity, entomopathogens cultivation on vegetable wastes and wheat bran can be attained by SSF system.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Beauveria, Metarhizium, Inoculation, Optimization and Conidia |
| Subjects: | G Geography. Anthropology. Recreation > GE Environmental Sciences Q Science > QH Natural history > QH301 Biology Q Science > QK Botany Q Science > QR Microbiology S Agriculture > S Agriculture (General) |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 18 Jun 2018 11:37 |
| Last Modified: | 18 Jun 2018 11:37 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/4321 |
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