Sileshi, Abraham (2011) Anti-Inflammatory and Bacterial Effect of Arthrospira plathensis (Spirulina) on Schistosomiasis mansoni and Selected Human Bacterial Pathogens. Masters thesis, Addis Ababa University.
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Abstract
Arthrospira platensis (spirulina) is multicellular filamentous blue green alga that is abundantly found in lakes of high salinity in Ethiopia. Several studies have shown that it has importance in human health and nutrition. The health benefits of spirulina relies mainly on tits anti-inflammatory, anti- oxidant and antimicrobial properties. In the current study spirulina was studied for its anti-inflammatory and antimicrobial effects using change in size and number of S. mansoni induced granuloma in infected mice and inhibitions of known human bacterial pathogens (S. typhi, S. dysenteriae, E. coli, S. aureus, S. epidermidis, C. freundii, and P. aeruginosa) as parameters. S. mansoni is a parasitic helmintic infection with high public health importance next to malaria, HIV-AIDS and TB especially in sub Saharan countries. Chronic inflammatory response in response to S. mansoni egg trapped in liver tissue is the main pathology responsible for the morbidity and mortality associated with the disease. Methodologies used in the study involve S. mansoni infection of Swiss albino mice, treatment of experimental animals with spirulina at two doses 100 and 400 mg/kg through oral, rectal, and oral/rectal. Animal dissection and histological staining of the liver to measure granuloma diameter, and finally antibiotic sensitivity test using disk diffusion method was done. Independent T. test comparison of the measurements form the anti-inflammatory test showed that the size of granuloma around an egg from spirulina treated compared to controls was significantly reduced. In association with this, comparison between different treated groups showed that treatment through oral route at a dose of 400 mg/ kg scores the highest percentage reduction both in granuloma size (59.53%) and granuloma number (72%). Result from the antimicrobial study showed that all test organisms except S. typhi were susceptible for the water suspension and extracts of spirulina unlike that of the Methanol extract. The possible explanations are discussed. In conclusion and recommendation the anti-inflammatory and antimicrobial effect of spirulina are summarized and future direction of research indicated. In association with this spirulina’s role in child health care and fight against child malnutrition and morbidity due to infection is underscored.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Spirulina, S. mansoni, Granuloma, Anti-inflammatory, Antimicrobia |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology Q Science > QR Microbiology > QR180 Immunology R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 18 Jun 2018 12:57 |
| Last Modified: | 18 Jun 2018 12:57 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/4372 |
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