Yeshamebel, Alelegne Adugna (2016) Optimization of Growth Regulators on In-vitro Propagation of Moringa stenopetala from Shoot Explants. Masters thesis, Addis Ababa University.
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Abstract
This thesis was conducted at Addis Ababa University entitled with Optimization of Growth Regulators on in vitro Propagation of M. stenopetala from shoot explant. Moringa stenopetala belongs to flowering family Moringaceae and genus Moringa. It is often referred to as the East African Moringa tree because it is native only to southern Ethiopia and northern Kenya. The expansion of its cultivation and utilization throughout the world especially in Africa is becoming important. For such expansion, the existing propagation method is limiting, so it needs good propagation system to supply enough planting material with uniform genotype. Therefore, the main objective of this study was to optimize an in vitro shoot multiplication protocol for M. stenopetala by using shoot tip as explants. Shoots were sterilized and cultured on Muraghige and Skoog (MS) medium for in vitro shoot initiation. For multiple shoot induction, the explants were cultured on MS medium supplemented with different concentrations of kinetin (0.5, 1.0, 1.5, 2.0, 2.5 mg/l) along with Indole-3- butyric acid( IBA) or α -naphthalene acetic acid (NAA) (0.01, 0.1, 0.5mg/l) and maintained at 25 ± 2°C for four weeks. Rooting was achieved by culturing well developed shoots in half strength MS medium containing IBA (0.1, 0.5, 1.0, 1.5, 2.0 mg/l), NAA (0.1, 0.5, 1.0, 1.5, 2.0 mg/l) and 0.5 mg/l IBA in combination with NAA (0.1, 0.5, 1.0, 1.5, 2.0 mg/l). Statistical analysis revealed that there was significant difference among all treatments applied in both shoot multiplication and rooting experiments. Maximum number of shoots per explant (3.43±1.41) and 7.97±4.18 leaves per explant were obtained on MS medium containing 0.5 mg/l kinetin in combination with 0.01mg/l NAA. The highest mean number of roots per shoot (1.63±1.03) and mean root length (0.87±1.22 cm) were obtained on MS medium containing 1.0 mg/l NAA and 0.1 mg/l IBA alone respectively. After acclimatization, 76% plants survived in greenhouse. In general, using NAA along with kinetin for shoot multiplication was better than kinetin along with IBA and application of NAA alone at concentration of 1.0 mg/l and 1.0 mg/l NAA along with 0.5 mg/l IBA were more effective for root induction.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | In vitro propagation, Moringa stenopetala, rooting, shoot multiplication |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology Q Science > QK Botany S Agriculture > SB Plant culture |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 26 Jun 2018 08:26 |
| Last Modified: | 26 Jun 2018 08:27 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/4576 |
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