Chromosome Study of some Ethiopian Anuran Amphibians

Tsige, Andualem (2008) Chromosome Study of some Ethiopian Anuran Amphibians. Masters thesis, Addis Ababa University.

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Eight families consisting of 24 genera and 63 species of amphibians have been recorded in Ethiopia, and out of these 40% are endemic. Little is dealt about the ecology and behaviour of amphibians, and karyotypes of amphibians in Ethiopia had not been studied prior to this study. Chromosome numbers and karyotypes are reported for eight species of anuran amphibians belonging to five genera, collected from various geographical locations in Ethiopia. Chromosome preparations were made from intestinal epithelial cells after pre-treatment with colchicin. Air-dried slide preparation was made by splashing techniques after treating the cells with 0.075 M KCL hypotonic solution for about 25 - 30 minutes followed by several rounds of centrifugation in fixative (3 methanol: 1 acetic acid). Chromosome characteristics were determined from mitotic metaphase chromosome spreads following chromosome staining with Giemsa in phosphate buffer (pH = 6.8). The result showed that four species: Ptychadena anchietae (Bocage 1867), Ptychadena neumanni (Ahl 1924), Ptychadena cooperi (Parker 1930), Hyperolius; have 2n = 24; two species Bufo, Bufo garmani Meek 1897, and B. regularis Reuss 1834, have 2n = 20; one species of Rana; Rana angolensis Bocage 1866 has 2n = 26; and a species of Xenopus, Xenopus clivii Peracca 1898 has 2n = 36. All species under the study are dominantly bimodal karyotype, i.e., composed of distinctly large and small chromosomes, except Xenopus clivii that varies in a gradual manner with most of sm and st chromosomes of the set. In the present study, different species of a given genera have very similar karyotype so that it is impossible to differentiate the species only on the basis of their karyotype.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Asymmetrical, Chromosome, Cytogenetic, Karyotype, and Symmetrical.
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Q Science > QL Zoology
Divisions: Africana
Depositing User: Selom Ghislain
Date Deposited: 16 Aug 2018 09:19
Last Modified: 16 Aug 2018 09:19

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