Evaluation of the Diuretic Activity of the Aqueous and 80% Methanolic Extracts of the Leaves of Ajuga Remota B. in Mice

Hailu, Wubshet (2011) Evaluation of the Diuretic Activity of the Aqueous and 80% Methanolic Extracts of the Leaves of Ajuga Remota B. in Mice. Masters thesis, Addis Ababa University.

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Abstract

In the Ethiopian traditional medicine the leaves of Ajuga remota (Local name ‘Armagusa’) is used as diuretic agent. Since the diuretic activity of this plant had not been investigated in scientifically controlled studies, the aim of the present study was to evaluate the diuretic potential of the aqueous and hydroalcoholic extracts of the leaves of A. remota in mice after acute oral administration. Adult mice were administered orally either aqueous (250 mg/kg, AA250; 500 mg/kg, AA500 and 1000 mg/kg, AA1000) or 80% methanolic (250 mg/kg, AM250; 500 mg/kg, AM500 and 750 mg/kg, AM750) extract and urine output and electrolyte contents were quantitated up to 5 h and compared with those administered with furosemide 10 mg.kg (F10) and distilled water (CON). The hydroalcoholic extract increased diuresis significantly (p<0.01) only at the maximum dose, while the aqueous extract increased diuresis at moderate (AA500, p<0.01) and higher (AA1000, p<0.01) doses by the end of the fifth hour compared to CON. Results on electrolyte excretion showed both the aqueous and hydroalcholic extracts had an increased natriuresis at the maximum doses (p<0.001 for AA1000 and p<0.01 for AM1000), while the kaliuresis effects were smaller at those doses when compared with standard, thus the plant had a beneficial potassium saving effect at these doses. Phytochemical screening of both extracts revealed the presence of secondary metabolites like phenolic compounds, tannins, saponins, flavonoids, terpenoids, steroids, and cardiac glycosides which might account for the diuretic activity. The plant was also found to be safer at a maximum dose of 5000 mg/kg for both extracts. In conclusion the results indicated significant diuretic activity at different doses and thus provide evidence for its folkloric use. And the different components like flavonoids are thought to be responsible for the evidenced diuretic activity.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Furosemide, Diuretics, A. remota, Natriuresis, Kaliuresis
Subjects:	Q Science > Q Science (General) R Medicine > RM Therapeutics. Pharmacology
Divisions:	Africana
Depositing User:	Vincent Mpoza
Date Deposited:	21 Jun 2018 07:37
Last Modified:	21 Jun 2018 07:38
URI:	http://thesisbank.jhia.ac.ke/id/eprint/5322

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