Study on Maize Pollen Volatile Compounds as Potential Attractants to Female Anopheles Arabiensis (Diptera: Culicidae) under Laboratory Condition

Wondwosen, Betelehem (2009) Study on Maize Pollen Volatile Compounds as Potential Attractants to Female Anopheles Arabiensis (Diptera: Culicidae) under Laboratory Condition. Masters thesis, Addis Ababa University.

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Abstract

This study on main Ethiopian vector of malaria, Anopheles arabiensis was designed to identify potential attractant volatile compounds from two differentially attractive strains of maize pollen, BH660 and ZM-521. The behavioral responses demonstrated by blood fed and unfed adult female An. arabiensis to odor extracts from BH660 and ZM-521 illustrated olfactory mediated attraction to both strains of maize pollen. The mosquitoes were attracted to the odor extracts from both strains differently: blood fed mosquitoes were attracted to lower doses of BH660 than to ZM-521 volatile extracts; whereas unfed females were attracted to lower doses of ZM-521 than to BH660 volatile extracts. Odor collections from these maize varieties were analyzed with both the gas chromatograph (GC) coupled to the mass spectrometer (MS) and the GC coupled to electroantennal detection (GC-EAD). These analyses showed that the antennae of both blood fed and unfed female An. arabiensis respond to volatile compounds extracted from both BH660 and ZM-521 pollens. Compounds determined from BH660 pollen to be active by GC-EAD were putatively identified as tetrahydro- 2, 5-dimethyl furan, nonane, decane, undecane, limonene, dodecane, 2-hexanol, ocyl acetate and decane, toluene, 3-hexanone, limonene, 2-hexanol, tridecane for blood fed and non blood fed respectively. Similarly, different compounds were identified from ZM-521 maize pollen, namely: nonane, 3-hexanone, dodecane, 2-hexanol, styrene, 2- heptanol, octyl acetate, 1-octen-3-ol, Zingiberene for blood fed and 3-hexanone, 2- hexanol, styrene, octyl acetate, 1-octen-3-ol, Zingiberene for blood unfed An. arabiensis. These results from the study showed that olfactory cues were the driving external factor that assisted both blood fed and nonblood fed An. arabiensis to inhabit areas near BH660 and ZM-521 maize pollen.

Item Type: Thesis (Masters)
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
Q Science > QL Zoology
S Agriculture > S Agriculture (General)
S Agriculture > SB Plant culture
Divisions: Africana
Depositing User: Selom Ghislain
Date Deposited: 20 Sep 2018 12:23
Last Modified: 20 Sep 2018 12:23
URI: http://thesisbank.jhia.ac.ke/id/eprint/5335

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