Tegegne, Biruk Getenet (2012) Genetic Diversity of Wild Coffee (Coffea arabica L.) Populations from Southwestern Ethiopia as Revealed by ISSR Markers. Masters thesis, Addis Ababa University.
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PDF (Genetic Diversity of Wild Coffee (Coffea arabica L.) Populations from Southwestern Ethiopia as Revealed by ISSR Markers)
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Abstract
Coffea arabica L., is indigenous to the highland of southwestern Ethiopia and southeastern Sudan. It is the afromontane rain forest species of south west and south east part of Ethiopia and grown naturally at altitudes ranging from 1,300 and 1,800 meter above sea level. Human population pressure and land use change in and around the afromontane rain forest highly threatened the wild coffee populations of Ethiopia. Inter simple sequence repeats (ISSRs) molecular markers were analysed. In the present study five wild populations of Coffea arabica from, Bench Maji-1(Maji), Bench Maji-2(Jeba), Dawero Banja, Limmu and Anfilo were included. A total of 71 individual plants were analyzed using four ISSR primers, three dinucleotides (810, 812,818) and one penta nucleotides (880) primers. A total of 30 clear and reproducible bands were amplified, and 100% were found to be polymorphic. The highest polymorphic loci were revealed by primer 812 with 9 polymorphic loci. Among the populations studied, Bench Maji-1 and Dawero showed 100% polymorphism, while Limmu and Anfilo showed moderate polymorphism of 56.67% and 63.33%, respectively, whereas Bench Maji- 2showed the least polymorphism(30%). The average gene diversity relative to the overall population was 0.45. AMOVA without grouping revealed that higher percentage of variation (79.4%) is attributed to the within population variation while the remaining variation is due to the among population variation (20.6%). Forest based UPGMA analysis based on Jaccard’s coefficient revealed one major group with Limu and Anfilo and two outliers (Bench Maji and Dawero Banja). NJ analysis based on individuals of wild Coffea arabica showed two distinct major clusters and sub-clusters within both major clusters; and individuals from Bench Maji-1 and Anfilo tend to form their own separate groups while others tends to speared all over the tree. In 3D PCO, most of Dawero Banja and Bench Maji individuals were intermixed with each other and made separate group from the other two populations but individual of Anfilo and Limmu were spread all over the plot.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Coffee, Coffea arabica, genetic markers, genetic diversity and ISSR |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 24 Sep 2018 12:48 |
| Last Modified: | 24 Sep 2018 12:48 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/5522 |
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