Chala, Adugna (2011) Antidiarrheal and Antispasmodic Activities of Essential Oil of Myrtus Communis L. Masters thesis, Addis Ababa University.
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Abstract
The essential oil of Myrtus communis (EOMC) was evaluated for its antidiarrheal and antispasmodic potential against isolated guinea ileum (GPI), ex-vivo antispasmodic model; normal and castor oil-induced intestinal transit in mice; castor oil-induced diarrhea in mice and prostaglandin induce enteropooling in rats. Atropine was used in GPI and normal intestinal transit test as a positive control, whereas loperamide was used in the castor oil-induced intestinal transit and castor oil-induced antidiarrheal test. EOMC inhibited normal intestinal transit siginificantly (p<0.05) and the effect was comparable with that of atropine. All doses (100, 200 and 400 mg/kg) of the oil employed showed significant antidiarrheal and antienteropooling activities which was comparable with that of the positive control loperamide. Different concentrations of the essential oil were used in the presence of agonist (Acetylcholine) in GPI as contraction stimulator in ex-vivo. The oil exhibited significant reductions in Acetylcholine-induced contractions of GPI. The agonist-induced contractions of GPI were greatly reduced by both doses of 50 μg/ml (p<0.01) and 100 μg/ml (p<0.01), suggesting a powerful spasmolytic property of the oil. The effect produced showed that the oil is much more efficacious than atropine (6.66 x10-9 M) in ex-vivo model. The oil appears to be more efficacious in ex-vivo than in vivo which may be due to the difference in physiological conditions that exists between the two systems. This study suggested that the essential oil of M. communis possesses spasmolytic and antidiarrheal properties which are likely to be due to the α-pinene and linalool present in the oil. The spasmolytic and antidiarrheal mechanisms might be in part mediated via Ca+-channel blockage. The results obtained in this study also support the traditional use of the plant for stomach pains, and diarrhea. However, further study should be conducted in order to determine the exact mechanism (s) of action of the oil and also to characterize the constituents responsible for the activity observed.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | M. communis, essential oil, antidiarrheal, antispasmodic, antienteropooling |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica |
| Divisions: | Africana |
| Depositing User: | Vincent Mpoza |
| Date Deposited: | 21 Jun 2018 14:18 |
| Last Modified: | 21 Jun 2018 14:18 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/5540 |
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