Wolde, Tesfaye (2010) Evaluation of Hepatoprotective Activities of Satureja Punctata Benth Briq and Solanecio Angulatus Vahl Jeffrey in Ferric Nitrillotriacetate Induced Hepatotoxicity in Rats. Masters thesis, Addis Ababa University.
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Abstract
The present study evaluated the in vivo hepatoprotective activity of two medicinal plants, namely, Satureja punctata Benth. Briq. (Lamiaceae) and Solanecio angulatus Vahl Jeffrey (Asteraceae) used in Ethiopian traditional medical practices for the treatment of liver diseases. The levels of hepatic marker enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were used to assess their hepatoprotective activity against ferric nitrilotriacetate (FeNTA)-induced hepatotoxicity in Sprague Dawely rats. The results revealed that pretreating rats with the crude aqueous extract of S. punctata at a dose of 500 mg/kg significantly suppressed the plasma AST (P < 0.01); ALT (P < 0.01) and ALP (P<0.001) activity when compared with control, while S. angulatus at a dose of 200 mg/kg reduced the activity of AST (P<0.01) and ALP (P<0.01) but failed to significantly suppress ALT activity. Among the soxhlet fractions of S. punctata, the methanol fraction at a dose of 500 mg/kg showed maximum hepatoprotective activity (P<0.001). The chloroform fraction at a dose of 250 mg/kg showed significant reduction in AST, ALT and ALP (P<0.01) activity while the aqueous fraction at a dose of 250 mg/kg showed significant reduction in AST (P<0.001), ALT (P<0.001) and ALP (P<0.05). Histopathological studies also supported these results. In vitro DPPH assay conducted on the soxhlet methanolic fraction of S. punctata showed that it possessed significant free radical scavenging activity which led to the conclusion that the hepatoprotective activity of the plant could be at least in part through its antioxidant action. The extracts showed good toxicity profile in acute toxicity study. Phytochemical analysis of S. punctata showed the presence of flavonoids, polyphenols, alkaloids and tannins. These results suggest that the aerial part of S. punctata contains bioactive principles with hepatoprotective activity.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Fe-NTA, S. punctata, S. angulatus, hepatoprotective, antioxidant |
| Subjects: | Q Science > Q Science (General) R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Africana |
| Depositing User: | Vincent Mpoza |
| Date Deposited: | 22 Jun 2018 08:17 |
| Last Modified: | 22 Jun 2018 08:17 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/5557 |
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