Blen, Weldegebreal (2016) Development of New Analytical Methods for the Determination of Caffeine Content in Aqueous Solution of Green Coffee Beans. Masters thesis, Addis Ababa University.
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PDF (Development of New Analytical Methods for the Determination of Caffeine Content in Aqueous Solution of Green Coffee Beans)
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Abstract
In the present work direct determination of caffeine in aqueous solution of green coffee bean was performed by using FT-IR-ATR and fluorescence spectrophotometers. In all the experiments water which is environmentally friendly and easily available everywhere was used for the extraction of caffeine from coffee beans. Experiments were performed without suffering from expensive and toxic chemicals. Caffeine was also directly determined in DMF solution using NIR spectroscopy with univariate calibration technique. This method can be used as alternative with reduced amount of organic solvent. The percentage of caffeine for the same sample of green coffee bean was determined using the three methods. The caffeine content of the green coffee bean in % w/w has been found to be 1.52 ± 0.09 using FT-IR-ATR, 1.50 ± 0.14 using NIR and 1.497 ± 0.05 using fluorescence spectroscopy. The means of the three methods were also compared by applying one way analysis of variance (ANOVA) and based on the ANOVA result at 0.05 significance level the means were not significantly different. The percentage of caffeine in green coffee bean was also determined by using previously developed UV/Vis spectrophotometric method for comparison. The caffeine content of the green coffee bean in % w/w was found 1.397 ± 0.02 using UV/Vis spectrophotometer for the same coffee sample.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | green coffee beans, caffeine, optical methods, FT-IR-ATR, NIR, fluorescence spectroscopy |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QK Botany |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 24 Sep 2018 13:27 |
| Last Modified: | 24 Sep 2018 13:27 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/5576 |
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