Tsegaye, Daniel (2016) Effect of Particle Size Reduction and Xylanase Cellulase Treatment of Wheat on Rat Hemoglobin Regeneration Efficiency. Masters thesis, Addis Ababa University.
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Abstract
Iron deficiency is probably the most common nutritional deficiency present in the world population. Predominantly plant-based diets that are constituted of wholegrain cereals and legumes contain non-heme iron that is poorly absorbed due to susceptibility to mineral chelating agents such as phytate and polyphenols, all of which may adversely affect mineral bioavailability. When attempts are made to remove or minimize the effects of the anti-nutrients, the dietary fiber matrix is often altered or destroyed, which would make it possible to better target the inhibitors and to increase their degradation. The objective of this study was to investigate the effect of enzymatic treatment and particle size reduction on and evaluate their contribution to the bioavailability of iron using hemoglobin regeneration efficiency of rats. Thirty two (21-28 days old) male Wistar rats were fed with an iron-free diet for 21-28 days to induce anemia. Then the rats were divided into four groups (n=8 per group) and fed with a diet which provides 35 mg of iron per kg. Hb measurement was done by HemoCue. Hematologic indices were calculated and bioavailability of iron was determined. The hemoglobin regeneration efficiency (HRE), was significantly higher (P< 0.05) in the control group, ferrous sulfate, n=8 (46.93±4.22), followed by the xylanase-cellulase treated (37.84±3.99), followed by the micro-milled (31.75±3.62) and wholegrain (23.66±4.51) wheat flour groups. The HRE relative to ferrous sulfate group (control 100%), of xylanase-cellulase treated wheat flour was 81.56±14.02%, micro-milled wheat flour was 68.45±12.23% and wholegrain wheat flour was 50.64±9.64%. The present in vivo study clearly showed that the treatments can lead to hemoglobin regeneration in iron deficient rats and is thus an important source of bioavailable iron.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Xylanase, Cellulase, Iron, Fiber, Wheat, Bioavailability, Hemoglobin Regeneration Efficiency, Relative Biological Value |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 25 Sep 2018 09:51 |
| Last Modified: | 25 Sep 2018 09:51 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/5714 |
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