Tsegaw, Dawit (2009) Isolation of Bioactive Compounds from a Forest Litter Decomposing Microfungus. Masters thesis, Addis Ababa University.
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PDF (Isolation of Bioactive Compounds from a Forest Litter Decomposing Microfungus)
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Abstract
In a screening of fungi isolates associated with litter decomposition (litotrophic) for bioactive metabolite production, LDMFS JP4 was isolated. It was identified to genus level using morphological (cultural) and microscopic characterization to belong to Geotrichum sp. A bioactive metabolite effective against bacteria and fungi, was isolated from this fungus at stationary phase of mycelium culture growing in solid substrate, rice. Through, bioassay guided fractionation of the crude extract using chromatographic methods, the active compound was isolated in pureform. The compound was found to exhibit activity. It was effective against gram positive (S. pnumoneae, S. aureus and B. subtilis) and gram negative bacteria (P. aeruginosa, E. coli and S. boydii) and the yeast, Candidia albicans. The active compound exhibited potent invitro antibacterial with a minimal inhibitory concentration (MIC) of 65-75μg/uL against Grampositive bacteria and 75-80ug/uL against Gram-negative. It was less effective for the yeast, Candidia albicans. The pure compound is promising and further work on chemical characterization is recommended.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | LDFs, taxonomic studies, solid substrate, bioactive metabolite, fractionation, minimal inhibitory concentration |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology Q Science > QK Botany S Agriculture > SD Forestry |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 01 Oct 2018 13:01 |
| Last Modified: | 01 Oct 2018 13:01 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/5771 |
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