Laser Trapping Ionization of Human Red Blood Cells with Four Hemoglobin Types: A Preliminary Study of Hemoglobin Quantitation

Deresse, Ahmed Adem (2017) Laser Trapping Ionization of Human Red Blood Cells with Four Hemoglobin Types: A Preliminary Study of Hemoglobin Quantitation. Masters thesis, Addis Ababa University.

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Abstract

In this work, a high intensity gradient laser was used to study the threshold ionization energy, the threshold radiation dose, and the charge (to determine hemoglobin quantitation) of four different samples of hemoglobin type. The study was conducted using AS, AC, FA or AF, and FSC hemoglobin types were obtained from MSCC at the MMC. The experiment was performed for each cell, for a total of 62 cells for Hb AS, Hb FA, and Hb FSC, and 47 cells for Hb AC, were trapped and ionized by a high intensity infrared laser at 1064 nm. With the laser trap serving as a radiation source, the cell underwent dielectric breakdown of the membrane. When this process occurs, the cell becomes highly charged and its dielectric susceptibility changes. The charge creates an increasing electrostatic force while the changing dielectric susceptibility diminishes the strength of the trapping force. Consequently, at some instant of time the cell gets ejected from the trap. The time inside the trap (ionization time) while the cell is being ionized is used to determine the threshold ionization energy and threshold radiation dose, and the intensity of radiation and the post ionization trajectory of the cells are used to determine the the charge for each cell of four different samples of hemoglobin type using NonlinearModelFit in Mathematica. Laser tapping technique is indeeded promissing for a very precise measurement of the hemoglobin types present in a blood sample. Knowing the hemodlobin type present in a blood sample is essential in screening sickle cell diseases and will vastly improve the accuracy of monitoring a sickle cell anemia patients receiving various types of treatments,

Item Type:	Thesis (Masters)
Subjects:	Q Science > Q Science (General) Q Science > QC Physics R Medicine > R Medicine (General)
Divisions:	Africana
Depositing User:	Selom Ghislain
Date Deposited:	04 Oct 2018 09:35
Last Modified:	04 Oct 2018 09:35
URI:	http://thesisbank.jhia.ac.ke/id/eprint/5933

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