Fikiru, Edossa (2006) Morphological and Molecular Diversity in Ethiopian Lentil (Lens Culinaris Medikus) Landrace Accessions and their Comparison with some Exotic Genotypes. Masters thesis, Addis Ababa University.
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PDF (Morphological and Molecular Diversity in Ethiopian Lentil (Lens Culinaris Medikus) Landrace Accessions and their Comparison with some Exotic Genotypes)
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Abstract
Seventy landrace accessions and eleven genotypes were used for morphological and ISSR diversity study. The plant materials were grown in a simple lattice design of two replications for morphological diversity study at Sinana Agricultural Research Center. Ten plants were selected randomly from each accession/genotype and marked before flowering. Morphological data were recorded from all selected plants on eleven traits. DNA was extracted from a bulk sample of five plants using a triple CTAB extraction technique. Molecular data were generated using four ISSR primers. ANOVA of morphological data indicated significant differences among the plant materials. Estimates of phenotypic and genotypic coefficients of variation showed wider variability within and between the landrace populations and between landraces and exotic genotypes for different morphological characters. Morphological dissimilarity between the populations ranged from 0.0519 to 0.73. Clustering analysis based on morphological dissimilarity matrix revealed three groups with distinctive morphological traits. The wider phenotypic variability observed encourages improvement activities between the lentils. Molecular analysis, on the other hand, showed that exotic genotypes were more diverse compared to the landraces. Genetic diversity within the total landrace population (HT) was 0.1734. High AR-based genetic diversity (0.1395 – 0.1039) was observed for samples from Gonder, Shewa and Wello. High within GR diversity was obtained for samples from NEE and the least for those from SEE. Exotic genotypes were more distantly related with samples from Tigray, Gojam and Shewa than to others. The genetic distances between populations of Ethiopian lentil landraces ranged from 0.2284 – 0.012. Samples from CE were more distantly related to samples of SEE, while the distance between other GRs was relatively low. Estimates of population differentiation and gene flow were moderately high for ARs. On the other hand, GR-based population differentiation was low, whereas among GR gene flow was high relative to AR-based population differentiation. AMOVA revealed higher within population variation than among population. Cluster analysis based on Dice’s similarity coefficient revealed three groups with distinct molecular profiles both with and without exotic genotypes. There were highly significant (p < 0.001) correlations, r = 0.45 and r = 0.26, between morphological and molecular distance matrices, respectively, of the entire plant materials and landraces separately, implying the validity of both methods for diversity study in lentils. Observation of low within AR/population diversity invites further collection activities, while enriching populations with high genetic diversity.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 27 Jun 2018 13:54 |
| Last Modified: | 27 Jun 2018 13:54 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/6048 |
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