Species Typing of Leishmania Isolates from Cutaneous Lesions of Patients in Ethiopia

Gadisa, Endalamaw (2005) Species Typing of Leishmania Isolates from Cutaneous Lesions of Patients in Ethiopia. Masters thesis, Addis Ababa University.

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The various species of Leishmania cannot be distinguished morphologically, but the treatment of leishmaniasis depends on the specific causative species. Thus accurate and rapid diagnosis of the specific species of Leishmania should be available for effective treatment of patients. Identification of Leishmania species in humans, in insect vectors and reservoir hosts should be available before planning for large-scale epidemiological survey, control programs and possible vaccine and/or drug trials. Therefore, the aim of this work was to establish isoenzyme technique at AHRI as a gold standard for species identification and introduce PCR-methods for species-specific diagnosis of leishmaniasis directly from clinical materials. In this line, cultured parasites from 55 localized cutaneous leishmaniasis (LCL) and 3 diffused cutaneous leishmaniasis (DCL) cases), diagnosed at the All Africa Leprosy and Tuberculosis Research, Rehabilitation and Training Center (ALERT) and from Ochollo village were analyzed. Species typing with isoenzyme electrophoresis and PCR-based techniques showed that, in all the cases, L. aethiopica is the aetiologic agent. Moreover, there was no strain difference between those causing LCL and DCL. The comparison of the isoenzyme and the PCRbased species typing techniques confirmed that the intergenic transcribed spacer-1 polymerase chain reaction-restriction fragment length polymorphism technique alone could be used for species typing. The sensitivity and specificity of the technique shows its potential for species specific diagnosis of leishmaniasis from clinical samples avoiding the need for culture. Thus, effective species-specific treatment of patients, which is a requirement in leishmaniasis, could be effected in a timely manner.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Leishmania aethiopica, Isoenzyme, ITS1-PCR-RFLP, ALERT, Ochollo
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Q Science > QL Zoology
R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions: Africana
Depositing User: Selom Ghislain
Date Deposited: 29 Jun 2018 09:32
Last Modified: 29 Jun 2018 09:32
URI: http://thesisbank.jhia.ac.ke/id/eprint/6113

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