Mesenbet, Esubalew (2008) Chemometrics - Assisted Determination of Emtricitabine and Tenofovir Disoproxil Fumarate Combination in Commercial Tablets. Masters thesis, Addis Ababa University.
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PDF (Chemometrics - Assisted Determination of Emtricitabine and Tenofovir Disoproxil Fumarate Combination in Commercial Tablets)
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Abstract
In this thesis, derivative spectrophotometric and multivariate method of assaying truvada® combination tablets, which contain tenofovir disoproxil fumarate 300 mg, and emtricitabine 200 mg, is described. Emtricitabine, 4-amino-5-fluoro-1-[2-(hydroxymethyl)-1,3-oxathiolan-5-yl]- pyrimidin-2-one, and tenofovir DF, 1-(6-aminopurin-9-yl)propan-2-yloxymethylphosphonic acid, are anti-HIV drugs of nucleoside / nucleotide reveres transcriptase inhibitor class. Emtricitabine was determined at 294.5 nm using the "zero crossing" technique because the signals of tenofovir DF is zero at this wavelength. Tenofovir DF was determined at 270.5 nm by the same technique because emtricitabine have no contribution at this wavelength. Tenofovir DF was quantified at 249.5nm and 270.5 nm wavelengths by using derivative ratio technique because the signal of emtricitabine was zero at these wavelengths. Emtricitabine was quantified at 288.5 and 303.5 nm because the signal of tenofovir DF was zero at these wavelengths. By classical least square (CLS) and principal component regression (PCR) methods good reproducible and repeatable results were obtained. In choosing the optimal magnitudes for the simultaneous determination of both drugs, the following criteria were considered: the linearity, the intercept, the sensitivity, the degree of interference, the relative percent error, the relative recovery, and the reproducibility. The R2 values which are in excess of 0.999, which indicated the good linearity of the calibration graphs. Sample recovery rates were found satisfactory (almost all are > 99%) with relative standard deviations (RSD) of less than 5%. The mentioned chemometrics-assisted techniques were successfully applied to simultaneous determination of emtricitabine and tenofovir DF in the laboratory-prepared mixtures and commercial tablets with considerable precision and good recoveries.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > QD Chemistry R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 29 Jun 2018 13:30 |
| Last Modified: | 29 Jun 2018 13:30 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/6343 |
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