Longitudinal Bacteriology of Burn Patients at Yekatit 12 Hospital Burn Center, Addis Ababa, Ethiopia

Deribie, Awoke (2011) Longitudinal Bacteriology of Burn Patients at Yekatit 12 Hospital Burn Center, Addis Ababa, Ethiopia. Masters thesis, Addis Ababa University.

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Introduction: Burn is one of the most common, devastating and a very painful form of trauma. Significant burn injuries induce a state of immunosuppression that predisposes burn patients to infectious complications. Sepsis and the resultant multi-organ failure are the leading causes of death in intensive burn care units. Rapidly emerging nosocomial and community acquired pathogens and the problem of multidrug resistance necessitates periodic review of isolation outline and their antibiotic susceptibility pattern in burn centers. Objective: The aim of this study was to determine longitudinal bacteriology of burn patients at Yekatit 12 hospital burn center, Addis Ababa. Methods and materials: A prospective hospital based study was carried out from Dec 2010 to Feb 2011. Wound swab and blood samples were collected on 0, 7th, and 14th, days of hospital stay and processed by conventional culture and biochemical identification procedures. Once identified, isolates were tested against commonly used antibiotics in the hospital by Modified Kirby-Bauer disc diffusion method. Data was analyzed by SPSS version 17.0 for Windows. Results: Forty one patients were enrolled in the study from both burn unit (BU) and outpatient (OPD). The mean total burned surface area (TBSA) was 11.9%. From 104 pus cultures 101 isolates were identified during the study period. In the first pus culture S. aureus was predominated, 15(46.9%) but later on Pseudomonas spp was increasing. Both spp together accounted 87.1% of the total isolates. Regarding blood culture isolates there was no significant change on time. Of 92 blood cultures 15 gram positive isolates were recognized. Coagulase negative Staphylococci (CoNS) noted with the largest percentage, 8 (53.3%) followed by S. aureus 6 (40%). Staphylococcus aureus showed moderate degree of resistance to the commonly used antibiotics. Majority of isolates were susceptible for clindamycin and vancomycin. But Pseudomonas spp were resistant for most of antibiotics used in the hospital. However, it was found more sensitive to norfloxacine as it was evidenced by only 15% resistance. Conclusion: Every treatment facility has microorganisms unique to it and these might change with time. It is therefore of paramount importance to have an in-depth knowledge of the resident organisms and their antibiotic sensitivity pattern so that infection related morbidity and mortality will improved. The nature of microbial wound colonization and flora changes should be taken into consideration in empirical antimicrobial therapy of burned patients.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Burn, Sepsis, Time related bacterial colonization, Antibiotic resistance
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions: Africana
Depositing User: Vincent Mpoza
Date Deposited: 03 Jul 2018 07:36
Last Modified: 03 Jul 2018 07:36
URI: http://thesisbank.jhia.ac.ke/id/eprint/6465

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