In vivo Antihypertensive and in vitro Vasodilatory Effect of the Crude Extracts and Fractions of Moringa stenopetala Leaves in Rodents

Geleta, Bekesho (2015) In vivo Antihypertensive and in vitro Vasodilatory Effect of the Crude Extracts and Fractions of Moringa stenopetala Leaves in Rodents. Masters thesis, Addis Ababa University.

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Abstract

Moringa stenopetala is an edible plant that has been medicinally claimed as a remedy for treatment of hypertension, diabetes, malaria and stomach pain mainly in southern parts of Ethiopia. The aim of this study was to evaluate in vivo antihypertensive effect in fructose induced hypertensive male Wistar rats and in vitro vasodilatory effect in pre-contracted isolated guinea pigs thoracic aorta strip. Rats were randomly divided into groups; three controls (positive, negative and normal control group) and 12 treatment groups of six male Wistar rats (n=6). Treatment groups were given daily crude extract, fraction and residue (250, 500, and 1000 mg/kg) of M. stenopetala orally with DFructose (66% w/v ad libitum). Whereas, positive, negative and normal control groups received Captopril (20 mg/kg/day with 66% w/v D-Fructose ad libitum), only D-Fructose (66% w/v ad libitum) and distilled water ad libitum, respectively for 15 days. On the other hand, for the in vitro vasodilatory study guinea pig was sacrificed by gentle cervical dislocation and the thoracic aorta ring was removed, cut spirally and mounted in an organ bath containing 37oC maintained Kreb’s Henseleit physiological solution aerated with carbogen (95% O2 and 5% CO2) for experiment. The study showed that aqueous and 70% ethanol extracts a significant prevention in increment in systolic, mean and diastolic blood pressure in a dose dependent manner compared with negative and normal controls. The extracts also showed suppression in increment in a total cholestrerol, glucose and triglycerides plasma level compared with normal control. In addition, the oral daily administration of extracts produced a rise in liver enzymes but no effect on kidney metabolites compared with normal control group. All extracts showed a relaxation effect in pre-contracted isolated thoracic aorta of guinea pigs in dose dependent manner. The in vivo antihypertensive, antidyslipidemic and in vitro vasodilatory effect was significantly shown with aqueous extract followed by 70% ethanol extract. But the effect was low in experimental groups that took ethyl acetate fraction and aqueous residue of aqueous extract.

Item Type: Thesis (Masters)
Uncontrolled Keywords: antihypertensive, vasodilatory, in vivo, in vitro, Moringa stenopetala, antidyslipidemic
Subjects: Q Science > Q Science (General)
R Medicine > RM Therapeutics. Pharmacology
Divisions: Africana
Depositing User: Vincent Mpoza
Date Deposited: 04 Jul 2018 08:35
Last Modified: 04 Jul 2018 08:35
URI: http://thesisbank.jhia.ac.ke/id/eprint/6559

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