Characterization of Mycobacterial Isolates from Lymph Nodes of Patients with Tuberculous Lymphadenitis in Dera Woreda, North Showa, Ethiopia

Seyoum, Berhanu (2015) Characterization of Mycobacterial Isolates from Lymph Nodes of Patients with Tuberculous Lymphadenitis in Dera Woreda, North Showa, Ethiopia. Masters thesis, Addis Ababa University.

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Abstract

Tuberculosis is a major health problem throughout the world causing a large number of deaths, more than that from any other single infectious disease. Extra pulmonary tuberculosis can occur in isolation or along with a pulmonary focus as in the case of patients with a disseminated form of tuberculosis. Tuberculous lymphadenitis (TBLN) is a common form of extra pulmonary tuberculosis. Lymph nodes (cervical, axillary and inguinal) are the most common sites of involvement. Diagnosis of TBLN is a formidable challenge in developing countries where there is high rate of human immunodeficiency virus infection. The aim of this study was to identify the etiological species of Mycobacteria responsible for TBLN in Derra, rural Ethiopia, where the status of TBLN was not known. A total of 153 patients who fulfilled the inclusion criteria were included in the present study during the study period between January 2004 and February 2005. Out of 153 study participants, fine needle aspirate specimens from 145 study participants were processed and analyzed using Ziehl Neelsen staining, culture, cytology and polymerase chain reaction. Out of 145 FNA samples, 66 (45.5%) of the isolates demonstrated growth on Lowenstein - Jensen medium. Among 145 clinically suspected TBLN patients, 115 (79.3%) were confirmed as TBLN cases by the combined results of AFB smear examination, culture, cytology and PCR. Two primer sets were used to identify the etiological agent at the complex (targeting the IS6110 insertion sequence) and species level (targeting the pncA gene allelic variation). From a total of 145 clinically suspected TBLN cases, 108 (75%) were positive by PCR at the complex levels. Based on PCR for detection of allelic variation at position 169, 107/108 (99.1%) were positive for M. tuberculosis and only 1(0.9%) was positive for M. bovis. The results indicated that M. tuberculosis is the causative species for tuberculous lymphadenitis in Dera.

Item Type: Thesis (Masters)
Subjects: Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
R Medicine > R Medicine (General)
Divisions: Africana
Depositing User: Vincent Mpoza
Date Deposited: 04 Jul 2018 10:30
Last Modified: 04 Jul 2018 10:30
URI: http://thesisbank.jhia.ac.ke/id/eprint/6610

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