Mekonen, Ketema (2015) Evaluation of the Acute and Subacute Toxicity of Aqueous Leaves Extracts of Artemisia Afra on Brain, Heart and Suprarenal Gland in Swiss Albino Mice. Masters thesis, Addis Ababa University.
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Abstract
Having primary health care is a human right which is fulfilled by western country because of expansion of health infrastructure, and increased quantity and quality of health professionals. But many developing countries including Ethiopia are yet far from achieving this. In Ethiopia, the majority of population relay on traditional medicine as a source of health care. The most common sources of traditional medicine are plants. A.afra is one of these plants that are used to treat different aliments. The aim of the present study was to investigate the toxic effects of A.afra on brain, heart and suprarenal glands. The study was conducted at Addis Ababa University, College of Health Science, School of Medicine, Department of Anatomy & Department of Physiology from Jan, 2014 to July, 2015. The plant was collected from Bale National park in Oromia Regional State. The plant was air dried and aqueous extract was prepared. In this research a total of 54 male and female mice of 8-12 weeks of age weighing 25-30g were used. The extract was given by oral rout in both acute and subacute study. The doses for acute toxicity study were 200mg/kg, 700mg/kg, 1200mg/kg, 2200mg/kg, 3200mg/kg, 4200mg/kg and 5000mg/kg of body weight, while for subacute toxcicy study a doses of 600mg/kg(low dose) and 1800mg/kg(high dose) of body weight were used. LD50 was grounded to be greater or above 5000mg/kg which indicates that the plant is relatively safe. There were no observed signs of toxicity at the lower three doses, although mild toxicity sign was observed at the higher dose level in dose dependent manner. In the subacute study, two treatment groups 600mg/kg and 1800mg/kg and one control group containing both sexes were used. Weights of mice were measured weekly and individual mice were observed for possible toxicity sign. At the end of 28 days, the animals were scarified and organs were harvested and processed for microscopic examination. No toxicity signs were observed in all treatment groups. There were also no significant weight changes between the treated and control group. On microscopic examination of the brain, heart and suprarenal glands no sign of cellular injury was observed. From this study it can be concluded that A.afra is relatively safe in mice.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Traditional medicine, A.afra, Toxicity study, LD50, Histopathology |
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QM Human anatomy R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 04 Oct 2018 12:29 |
| Last Modified: | 04 Oct 2018 12:29 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/6718 |
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