Fekadu, Worku G/Mariam (2008) Chromosome Study of some Fish Species from Rift Valley Lakes and some Other Water Bodies of Ethiopia. Masters thesis, Addis Ababa University.
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Abstract
Fishes are the most diverse of the major vertebrate groups. However, cytogenetic studies in fish have not been well studied globally as well as here in Ethiopia. Five species belonging to four families were sampled randomly from rift valley lakes and some water bodies; and their mitotic chromosomes have been studied or determined using splashing method. For chromosome preparation, each live fish specimen was injected with colchicine (0.5% of 0.8/100 gm body weight) and kept alive for 6-8 hours. The gill filaments were removed and kept in hypotonic solution (0.75M KCL) for about 30 minutes and then were fixed in 3:1(methanol: glacial acetic acid) for 30 minutes. Gill filaments were grinded with 1-1.5 ml of fixative solution and filtered with gauze. Finally the cell solution centrifugation for 5 minutes and air-dry slide preparation was made and stained in Giemsa. Photographs were taken from the best metaphase plates of each specimen and chromosome numbers were determined. The result showed that, the chromosome numbers of (2n) obtained for Tilapia zilli (2n=44), Oreochromis niloticus (2n=44), Clarias gariepinus (2n=56), Synodontis schall (2n=54), and Carassius auratus (2n= 94) all except the last fish specimen are in agreement with the previous reports. Since karyotypic analysis could not be given to all species under the present study, it is better to carry out further cytological study in the future to get more reliable chromosome data for better understanding about their taxonomic and evolutionary relationships.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Fish, Tilapia, Oreochromis, Clarias, Synodontis, Carassius, diploid chromosome number, Rift Valley lakes, Ethiopia. |
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QL Zoology |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 12 Oct 2018 12:15 |
| Last Modified: | 12 Oct 2018 12:15 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/6832 |
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