Nureye, Dejen (2017) In Vivo Anti-Malarial Activity of 80% Methanol Root Bark Extract and Solvent Fractions of Gardenia Ternifolia Schumach. & Thonn. (Rubiaceae) Against Plasmodium Berghei Infected Mice. Masters thesis, Addis Ababa University.
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Abstract
Irrespective to tremendous reduction in incidence and prevalence during the last decade, malaria continues to remain a major global public health problem. Spread and evolution of resistance to the available antimalarial drugs endanger all the recent gains in malaria control. This issue makes the development of novel drugs a necessity. The key source in search of such drugs is medicinal plants. Gardenia ternifolia is used by traditional healers in Africa including Ethiopia for managing malaria. However, no scientific investigations have been carried out to substantiate the claim. Thus, this study was conducted to evaluate in vivo antiplasmodial activity of 80% methanolic root bark extract and solvent fractions of the plant in mice model. To this effect, various doses (200, 400 and 600 mg/kg) of the extract and fractions were administered. Parameters, including parasitemia, survival time, body weight, temperature, and packed cell volume were then determined using suppressive, curative and prophylactic tests. Chemo-protective effect exerted by the crude extract and fractions ranged between 30-59% and 14-51%, respectively. The curative and prophylactic effects of the crude extract were in the range of 36-63% and 24-37%, respectively. All dose levels of the crude extract prevented loss of weight, reduction in temperature and anemia on early and established infection. Butanol and chloroform fraction did also reverse reduction in temperature, body weight and packed cell volume at different degree of significance. Phytochemical screening of the extracts showed the presence of different secondary metabolites. The results indicate that the plant has a promising antiplasmodial activity and it could be considered as a potential source to develop new antimalarial agents.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | In vivo, Anti-malarial activity, Gardenia ternifolia, Plasmodium berghei, Butanol fraction |
| Subjects: | Q Science > Q Science (General) R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica |
| Divisions: | Africana |
| Depositing User: | Vincent Mpoza |
| Date Deposited: | 18 Oct 2018 11:07 |
| Last Modified: | 18 Oct 2018 11:07 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/6876 |
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