Misganaw, Desye (2017) Evaluation of Anti-Malarial Activity Of 80% Methanol Extract and Solvent Fractions of the Leaves of Olea Europaea Linn (Oleaceae) in Mice. Masters thesis, Addis Ababa University.
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Abstract
Drug resistance posed a challenge to malaria control measures. This called for development and discovery of new chemotherapeutic agents from alternative sources such as medicinal plants. This study aimed to investigate antimalarial activity of Olea europaea, an extensively used plant in Ethiopian folklore medicine. The leaf was extracted by maceration using 80% methanol and the crude extract was then successively fractionated using solvents of differing polarity (chloroform, n-butanol and water). Antimalarial activity of various doses of the extract and fractions (200, 400 and 600 mg/kg) was evaluated using the 4-day suppressive test (both crude and fractions), as well as Rane’s and prophylactic tests (crude extract only). The crude extract significantly reduced parasitemia (p<0.001) and prolonged survival time (p<0.001) in all models, as compared to the negative control group. Accordingly, percentage suppression was highest on the 4- day suppressive test followed by the Rane’s test and then the prophylactic test. The nbutanol fraction produced the highest percentage inhibition of parasitemia among the fractions, while the aqueous fraction was the least and the rank was also similar for other parameters (packed cell volume, rectal temperature and body weight) as well. Hence, this study revealed the anti-malarial activity of the crude extract of the leaves of Olea europaea and further shown that all the three fractions possessed varying degree of antimalarial activity.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Antimalarial activity, Plasmodium berghei, Olea europaea |
| Subjects: | Q Science > Q Science (General) R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Africana |
| Depositing User: | Vincent Mpoza |
| Date Deposited: | 24 Oct 2018 08:25 |
| Last Modified: | 24 Oct 2018 08:25 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/6984 |
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