Kebede, Edosa (2016) Bacterial Profile and Antibiotic Sensitivity Pattern of the Isolates from Operating Room Environments in Government Hospitals in Addis Ababa, Ethiopia. Masters thesis, Addis Ababa University.
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Abstract
Background : Contamination of the operating theatre is a major cause of nosocomial infection. Bacterial pathogens isolated from hospital environments are also known to develop resistance to multiple antimicrobial agents. Objective: To assess the sanitation practices of Health Care Workers (HCWs) as well as bacterial profile and the antibiotic sensitivity pattern of the isolates in operating room environments of government hospitals in Addis Ababa. Methods: A hospital based cross sectional study was conducted from August to November 2014 in Government Hospitals in Addis Ababa. The total of 346 specimens of surfaces swabs and air samples were collected using sterile cotton tipped swabs moistened with normal saline and open plate technique. The study was also supplemented by observation and information gathered from HCWs. Data was analyzed by SPSS version 16 and the results were explained by using percentage, tables and different figures. Result: Of the 346 samples, 47.4% (n=164)were positive for bacterial growth, 23.8% (n=39) by open plate and 76.2% (n=125) by swabbing method, respectively. A total of 221 bacterial pathogens were recovered from all samples processed in this study. Coagulase negative Staphylococcus, 160(72.4%) were the predominant bacterial isolates followed by S. aureus 33(15%). Antibiotic susceptibility pattern of bacterial pathogens showed overall sensitivity of 88.7% to ciprofloxacin and most of them had overall resistance of 42.5% to tetracycline and 33.5% to chloramphenicol. A total of 89 healthcare workers were interviewed. Majority of them 58(65.2%) had training on hand hygiene practice. Sixty four percent 57(64.0%) of the respondents routinely used an alcohol-based hand rub for hand hygiene, 59(66.3%) had ever wore at least five types of personal protective equipment (PPE) always while providing care. Conclusion and Recommendation: In general, we found coagulase-negative staphylococci to be the major species contaminating the air and other surfaces in the operating rooms. This may be due to Staphylococci are usually human in origin and point to the restriction of traffic in operating rooms. Microbiological surveillance of operating theaters can play an important role in reducing bacterial contamination consequently preoperative infectious episodes can be reduced considerably.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > QR Microbiology R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Vincent Mpoza |
| Date Deposited: | 25 Oct 2018 07:49 |
| Last Modified: | 25 Oct 2018 07:49 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7013 |
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