Afework, Ejigayehu (2016) Agreement Between Enzyme Linked Immunosorbent Assay with Rapid Plasma Reagin and Treponemapallidumhaem Agglutination Assay for Screening and Diagnosis of Syphilis at National Blood Bank Service in Addis Ababa, Ethiopia. Masters thesis, Addis Ababa University.
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Abstract
Background: Serological diagnosis of syphilis is based on demonstration of specific treponemal antibody and non treponemal antibodies (reagin) in body fluids. Accurate and reliable testing is a critical step in ensuring the diagnosis of syphilis and the safety of blood transfusion service. Performance agreement between syphilis tests is not documented well in Ethiopia. Objectives: To determine agreement between Enzyme Linked Immunosorbent Assay with Rapid Plasma Reagin and TreponemapallidumHaemagglutination Assay for screening and diagnosis of syphilis. .Methods:Laboratory based Cross sectional study was carried out from January 2016 to June 2016 at National Blood Bank Service Addis Ababa, Ethiopia. All positive (One hundred and ninety) and 190 negative samples were used which stored in NBBS (National Blood Bank Service) laboratory from July 2015 to Dec 2015 (total 380 samples). Systematic random sampling method was used to select negative samples .The data was analyzed by SPSS version 20 software. The percentage agreement and κ value were compared; the sensitivity, specificity, positive predictive value and negative predictive value of the ELISA and RPR were calculated. P value less than 0.05 were taken as statistical significant. Results:From 190 ELISA positive sera, 151 (80%) were confirmed as positive by TPHA, 39 (20 %) were found to be false positive. 59 (39%) of samples were positive and 92 (61%) were false negative by RPR. From 190 ELISA negative sera all were negative by RPR and TPHA. The sensitivity, specificity, PPV and NPV of ELISA was 99.9%, 85%, 79 % and 100% respectively. While RPR was 62%, 99 %, 100% and 63 % respectively. Percent agreement of ELISA with TPHA was 90% and corresponding kappa value was substantial 0.795. Percent agreement for RPR was 66 % with kappa value of 0.375 which was a fair agreement.Chi-square result with p value 0.000 which is statistical significant indicate the association between categorical variables. Conclusion The study demonstrated that ELISA was very sensitive. There was good agreement between ELISA and TPHA. RPR was with high specific and weak sensitive results. There was poor agreement between RPR and TPHA.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine |
| Divisions: | Africana |
| Depositing User: | Vincent Mpoza |
| Date Deposited: | 25 Oct 2018 07:07 |
| Last Modified: | 25 Oct 2018 07:07 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7018 |
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