Debebe, Mekonnen (2014) Evaluations on the Sub-Chronic Toxicity of 70% Ethanolic Seed Extracts of Albizia Gummifera and Millettia Ferruginea on Blood Parameters and Liver and Kidney Tissues in Albino Wistar Rats. Masters thesis, Addis Ababa University.
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Abstract
Albizia gummifera and Millettia ferruginia are plants found in Ethiopia that have different medicinal values. The objective of the present study was to evaluate the toxicological effects of sub-chronic administered hydro-ethanolic (70%) seeds extract of Albizia gummifera and Millettia ferruginea in albino Wistar rats. The seeds of these plants were collected from different areas of Ethiopia. They were dried and crushed to powder and macerated with hydro-alcohol and placed in orbital shaker. The extract was then filtered through Whatman filter paper No.1 and the filtrate was evaporated to dryness by Rota vapor and further concentrated by water bath at 40 oC. The extract was packed in air tight brown glass bottles and kept in a refrigerator at 4 oC. The extract was then administered to rats at different doses to determine the LD50 of the extract and at doses of 125mg/kg/day and 250mg/kg/day for the sub-chronic toxicity study. The LD50 of Albizzia gummifera and Millettia ferrugina were found as 4000mg/kg and 3500mg/kg, respectively. Statistically significant difference in body weight was observed in female rats in the 10th week at 250mg/kg body weight of seeds extract of Albizia gummifera administered group and in the male rats at lowest dose during the 9th and 10th weeks of administration period for seeds extract of Millettia ferruginea. The seeds extract of Albizia gummifera statistically decreased (p ≤ 0.05) MCH in the male rats at both doses; MCHC at both 125mg/kg and 250mg/kg in the female rats; and MCH in the female rats at higher dose and increased RDW-CV in the male rats at both doses. It increased NEUT at the highest dose in both females and males. The seeds extract of Millettia ferruginea decreased (p ≤ 0.05) in the MCHC and MONO of female rats at the highest doses. ALP, ALT and urea were found significant in the female rats administered with 250mg/kg of Millettia ferruginea seeds extract. While, seeds extract of Albizia gummifera increased only urea in male rats at 250mg/kg. Some histopathological changes in liver and kidney were also observed for both plants extracts. There were inflammation, congestions and focal hepatocellular necrosis of the liver tissue. The extracts also produced atrophy of the glomeruli of the kidney. The observed changes in both of the plant seeds extract might have resulted because of the presence of some bioactive ingredients in the extract. Therefore, the active ingredients which might be responsible for toxic insult should be researched with their mechanisms of actions.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Albizia gummifera, Millettia ferruginea, seeds, hydroethanolic extract, toxicity, Wistar Albino Rats |
| Subjects: | Q Science > QM Human anatomy Q Science > QR Microbiology R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 29 Oct 2018 09:48 |
| Last Modified: | 29 Oct 2018 09:48 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7081 |
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