Gadisa, Eshetu (2015) Combined Antibacterial Effect of Essential Oils from Three Most Commonly used Ethiopian Traditional Medicinal Plants on Selected Multidrug Resistant Bacteria. Masters thesis, Addis Ababa University.
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PDF (Combined Antibacterial Effect of Essential Oils from Three Most Commonly used Ethiopian Traditional Medicinal Plants on Selected Multidrug Resistant Bacteria)
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Abstract
Background: An alarming increase of emerging and re-emerging of multidrug resistance (MDR) cause serious difficulties in treatment and continued to be public health problem globally. As a result, searching for bioactive with chemical diversity and broad spectrum from medicinal plants is priorities in curb life threaten infectious agents. Those properties made them candidate for treat Enterobacteriaceae and/or methicillin resistance Staphylococcus aureus. However, they have not been screened for their combined effect on multi-drug resistant (MDR) bacteria. Objective: The aim of this study was to evaluate single and combined antibacterial effect of essential oils obtained from Blepharis cuspidata, Boswellia ogadensis and Thymus schimperi against multidrug resistant human pathogen bacteria. Methodology: Essential oils obtained by steam distillation from aerial parts of twelve plants were evaluated for antibacterial effect. They were adsorbed to antibiotic discs and placed on MHA seeded with five MDR and reference strains. Only three of them, having greater than14mm inhibition zone diameter (IZD) were selected and evaluated for their combined effects. Then, their minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were measured in 1:1 ratio. Their interaction was determined by fractional inhibitory concentration (FIC) index. Results: Combined essential oil obtained from Blepharis cuspidata and Thymus schimperi had inhibition zone (39mm), MIC and MBC values (0.39mg/ml) against MRSA. This combination was also potent against gram negative MDR bacteria. They had inhibition zone ranging from 28-35mm and MIC and MBC were ranging (0.39-6.25mg/ml), (0.78- 12.5mg/ml) respectively. Moreover, combined effect of essential oils from Blepharis cuspidata and Boswellia ogadensis had inhibition zone (22-29mm). Their MIC value ranges 0.78-6.25mg/ml for Enterobacteriaceae (Escherichia coli (R), Klebsiella pneumonia (R), and Acinetobacter species (R). It had also potent against MRSA. Conclusion and Recommendation: Combined essential oils were found to have more antibacterial effect than using alone and even it has better inhibition zone diameter than tested modern drugs. Hence, it can be applied to a pharmaceutical composition as modulators or adjuvant or precursors for synthesis of new antibiotics in future activities.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Vincent Mpoza |
| Date Deposited: | 29 Oct 2018 13:42 |
| Last Modified: | 29 Oct 2018 13:42 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7104 |
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