Hussien, Mohammed (2014) Preparation of Collagen Hydrolysate Syntan from Delimed Pelt Trimmings for Post Tanning Application and for Stabilzing Collagen Fibers. Masters thesis, Addis Ababa University.
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Abstract
Delimed pelt trimmings are trimmings that are obtained from limed pelt trimmings by removing lime. Limed pelt trimmings are one of the solid wastes that are generated in leather processing which is common in Ethiopia unlike other countries. These wastes are important resources for producing collagen hydrolysate which can be applied in leather processing operations. The aim of this study is to prepare collagen hydrolysate from delimed pelt trimmings and to apply it in wet blue goat skin in post tanning operation to determine the optimum hydrolysis conditions and molecular weight of the collagen hydrolysate. And also to determine its stabilizing effect on collagen fibers by treating rat tail tendon with the collagen hydrolysates by carrying out enzymatic and thermal stability experiments. The optimum conditions of hydrolysis for post tanning operation was determined by preparing six different types of collagen hydrolysates and apply it post tanning operation as a retanning agent. Based on the absorption of chemicals and organoleptic properties, the best collagen hydrolysate is selected and characterized further using FPLC and MALDI. The results showed that collagen hydrolysates play a role in stabilizing collagen fibers. And also the best collagen hydrolysate for post tanning is obtained when the proportion of high molecular weight to low molecular weight proportion of the fractions is 80% to 20%. And the range of the molecular weight should be in between 2000 to 6000 daltons. This can be achieved when the hydrolysis condition is carried out by using 0.8% trypsin for duration of 3 hours. It is concluded that by preparing collagen hydrolysates syntans from delimed pelt trimmings satisfactory results can be achieved by applying it in post tanning operation during leather processing and also they can be used for stabilization of collagen fibers.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Chemical and Bio Engineering |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry S Agriculture > S Agriculture (General) T Technology > TP Chemical technology |
| Divisions: | Africana |
| Depositing User: | Andriamparany Edilbert RANOARIVONY |
| Date Deposited: | 31 Oct 2018 10:07 |
| Last Modified: | 31 Oct 2018 10:07 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7203 |
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