Teka, Frehiwot (2015) Hypotensive Activity Of 70% Ethanol Extract of Aerial Part of Ruta Chalpensis both In Vivo and Ex Vivo and its Antioxidant Activity. Masters thesis, Addis Ababa University.
![[img]](http://thesisbank.jhia.ac.ke/style/images/fileicons/application_pdf.png) |
PDF (Hypotensive Activity Of 70% Ethanol Extract of Aerial Part of Ruta Chalpensis both In Vivo and Ex Vivo and its Antioxidant Activity)
Firehiwot Teka.pdf - Accepted Version Restricted to Repository staff only Download (1MB) | Request a copy |
Abstract
Introduction:-Hypertension is a chronic elevation of systemic arterial pressure above a certain threshold value. It is a serious health problem worldwide that contributes to myocardial infarction, cerebrovascular accidents, and premature mortality. Medicinal plants are used to treat different diseases in different parts of the world. There are number of medicinal plants in the management of hypertension. One such plant is Ruta chalpensis which is used to treat different ailment in different parts of the world. Objective: - The objective of this study was aimed to screen the secondary metabolites and to identify whether the aerial part of crude extract of Ruta chalpensis alters blood pressure and its effect on reactive oxygen species. Materials and methods: -The study was conducted on twelve guinea pigs to measure the effect of Ruta chalpensis 70% ethanol extract by using invasive and organ bath methods. Qualitative chemical screening involving color change was applied to see the phytochemicals present in plant extract, and Spectrophotometric analysis by DPPH method has been used to see the antioxidant activity. Result:- The areal part of 70% ethanol extract of Ruta chalpensis was found positive for, major secondary metabolites, The IC50, value was computed to be 21.72mM of ascorbic acid and 58.20mM of 70% ethanol extract of areal part of Ruta chalpensis and an inhibition of 88.70% and 96.96% at 500ug/ml of the extract and the standard ascorbic acid respectively. After induction of aortic muscle contraction by high concentration of k+ the extract showed a significant (p<0.001) ability to relax the isolated aorta of guinea pigs. The extract caused significant (p<0.001) fall in systolic, diastolic, and mean arterial blood pressure (MABP), There were no significant differences (p=0.526) in pulse pressure. Conclusion: - This study justifies the traditional use of this plant for the treatment of hypertension and it can be additional source of natural antioxidant.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Hypertension; in vivo; ex- vivo; Ruta chalpensis Blood pressure; antioxidant; Phytochemical |
| Subjects: | Q Science > Q Science (General) R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine |
| Divisions: | Africana |
| Depositing User: | Vincent Mpoza |
| Date Deposited: | 19 Nov 2018 12:51 |
| Last Modified: | 19 Nov 2018 12:51 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7316 |
Actions (login required)
 |
View Item |