Abrar, Mohammed Mehdi (2017) Blood and Tissue Enzyme Activities of GDH, LDH, Index of Glutathione and Oxidative Stress among Breast Cancer Patients Attending Referal Hospitals of Addis Ababa, Ethiopia: A Comparative Cross Sectional Study. Masters thesis, Addis Ababa University.
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Abstract
Background: The exact cause of breast cancer is still unknown, however, it is a multifactorial and devastating disease. It is commonly diagnosed and second leading cause of death among women globally. Scholars are searching for an early marker for diagnosis, prognosis and potential target for treatment by looking it through many directions. One of the direction is its metabolism, all types of cancer need high energy input and substrates via aberrant metabolism for their survival. Therefore, the present study focused on investigating and examining pathways of glucose and glutamate metabolism and oxidative stress in breast cancer patients via analysis of glutamate dehydrogenase, lactate dehydrogenase, reduced and oxidized glutathione and the oxidative stress index of breast cancer patients. Methodology: Comparative cross-sectional study was conducted from July, 2015 to May, 2017. A total of 54 (27 breast cancer patients and 27 control groups) participants were recruited. All were age-sex matched and mean age of breast cancer patients were 44.93 (25–68), whereas mean age of controls were 45.2 (24-66). Result: Catalytic activities of glutamate dehydrogenase, lactate dehydrogenase and oxidative stress index were significantly increased both in serum (4.2mU/ml, 78.6mU/ml and 3.3:1 respectively) and cancerous tissues (1.4mU/ml), 111.7mU/ml and 2.15:1 respectively) of breast cancer patients as compared to serum of control groups (3.15mU/ml, 30.4mU/ml and 2.05:1 respectively) and non-cancerous tissue of breast cancer patients (0.92mU/ml, 70.5mU/ml and 1.1:1 respectively) (P≤0.05). Correspondingly, ratios of reduced to oxidized glutathione were significantly decreased both in serum (20:1) and cancerous tissues (23.5:1) of breast cancer patients when compared to serum of control groups (104.5:1) and non-cancerous tissues of breast cancer patient (70.9:1) (P≤0.05). Conclusion: Catalytic activities of glutamate dehydrogenase and lactate dehydrogenase, and ratios of total oxidative status to total antioxidant capacity were significantly increased among breast cancer patients when compared to serum of control groups and non-cancerous tissues of breast cancer patients. While, ratios of reduced to oxidized glutathione and total antioxidant capacity in serum and tumor tissues of breast cancer were significantly decreased as compared to serum of control groups and normal tissues of breast cancer patients.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Glutamate Dehydrogenase, Lactate Dehydrogenase, Reduced Glutathione, Oxidized Glutathione, Total oxidative status and Total antioxidant capacity and breast cancer |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology R Medicine > R Medicine (General) |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 20 Nov 2018 09:55 |
| Last Modified: | 20 Nov 2018 09:55 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7566 |
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