Assesment of Different Food Contaminant Bacterial Species and Their Antimicrobial Susceptibility Pattern among Some Street Vended Foods in Metema Yohannes Town, Amhara Region, North Gondar Zone, Ethiopia

Seid, Muhammed (2016) Assesment of Different Food Contaminant Bacterial Species and Their Antimicrobial Susceptibility Pattern among Some Street Vended Foods in Metema Yohannes Town, Amhara Region, North Gondar Zone, Ethiopia. Masters thesis, Addis Ababa University.

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Abstract

Background: Street vended foods are those foods prepared on the street and ready to eat, or prepared at home and consumed on the street without further preparation. The street food economy is a growing sector in many countries. In spite of numerous advantages offered by street vended foods, many evidence revealed that foods exposed for sale on the roadside are contaminated by either spoilage or pathogenic microorganisms. Methodology: A cross-sectional study was conducted from October 2, 2015 to December 30 2015 on street vended foods in Metema Yohannes Town, Northern Gondar Zone. 32 food samples from four different food items ie, (Sanbusa, Anbasha, Foul and Bonbolino) were analyzed for bacterial assessment & load. 25gm of each food sample was transferred in to 225 ml of buffered peptone water and homogenized. The homogenates were serially diluted to 10-6 and a volume of 0.1 ml dilution was spread on a Plate Count Agar, MacConkey Agar, Mannitol Salt Agar and Salmonella Shigella Agar and incubated for the Total Aerobic Plate Count, Enterobacteriaceae, Staphylococcal & Salmonella Shigella count respectively. Antimicrobial susceptibility test was done for isolated bacterial species. A structured questionnaire was used to collect data on Socio-demographic & hygienic condition of the vendors. Percentage and frequency of data was analyzed using SPSS version 21 software. Results: Two bacterial species, Staphylococcus aureus and Escherichia coli were mainly isolated. S. aureus was isolated from all 32 samples while E.coli was isolated from 28. The Aerobic bacterial count ranged from 2.1X105 CFU/g to3.9X106 CFU/g whereas the total Enterobacteriaceae and total Staphlococcal count ranged from 1.9X103CFU/g to 3.8X104 and 1.3X103 CFU/g to 1.3X105 CFU/g respectively. No Salmonella or Shigella was isolated. Ciprofloxacin and Norfloxacin were found to be the most effective antimicrobials against both S.aureus and E.coli isolates, Resistance was observed against Ampicillin for both E.coli and S.aureus whereas Tetracycline and Ampicillin were not effective against E coli. Conclusion: The study revealed that street vended foods that were sold at selected study sites of Metema Yohannes town do not meet the required quality and safety levels of ready to eat foods. They are contaminated with the disease causing bacteria mainly E.coli and S.aureus. The possible source of contamination may be the unhygienic manner of food during preparing, storage, handling and displaying for sell. Good implementation of food sanitation, health education, and personal as well as food hygiene & prevention of infection should be promoted for street food vendors.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	foods, Bacterial profile, Antibiotic Susceptibility Test. Metema Ethiopia
Subjects:	Q Science > QR Microbiology R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions:	Africana
Depositing User:	Emmanuel Ndorimana
Date Deposited:	21 Nov 2018 08:57
Last Modified:	21 Nov 2018 08:57
URI:	http://thesisbank.jhia.ac.ke/id/eprint/7606

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