Debelo, Nigatu (2015) The Histopathologic Effects of Thymus Serrulatus and Thymus Schimperi (Tosign) on Liver, and Kidney; and Blood Parameters of Mice and Rats Respectively. Masters thesis, Addis Ababa University.
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Abstract
Introduction: The World Health Organization (WHO) defines traditional medicine as health practices, approaches, knowledge and beliefs incorporating plant, animal and mineral based medicines, spiritual therapies, manual techniques and exercises, applied singularly or in combination to treat, diagnose and prevent illnesses and maintain well-being. More than 95% of traditional medical preparations are of plant origin. One of the widely used herbal medicinal plants is Thymus species. Materials and Methods: Laboratory based experiment was conducted using the aqueous extract of T.schimperi and T. serrulatus which were given using intragastric gavageto Wistar rats and Swiss Albino mice, respectively. All animals were obtained from Ethiopian Public Health Institute (EPHI). For acute toxicity study the LD50s was determined by using Protocol for LD50 determination.A total of five female animals were used for each dose level investigated. The following doses, 300mg/kg for group-1, 2000mg/kg for group-2, 5000 mg/kg for group-3, and 10,000mg/kg for group-4, were used. For sub-chronic study animals of the same sex were grouped into experimental and control groups for aqueous extracts of the plant. Each group of animal was given different doses of the aqueous extracts. These included treatment of one group with 200mg/kg and another with 600mg/kgfor 90 days. Animals in the control group received distilled water.Finally, the animals were sacrificed and blood samples were collected for hematological and biochemical analysis. Liver and kidneys were harvested for weight determination and were checked for gross pathological changes, and then processed for tissue section preparation and microscopic examination. Results: The current study found that the median oral lethal doses (LD50s) are higher than 10,000mg/kg for both plants. Generally in the acute toxicity study; the behavior, organ and body weights were not altered in mice and rats treated with doses up-to 10,000mg/kg. After 12 weeks of sub chronic treatment with aqueous extract of both plants, there was no significant change in body weight,and most of the evaluated hematological and biochemical parameters. There was a significant decrease in count of basophils in the female rats, at doses of 200 mg/kg (81%), in male rats treated at doses 200mg/kg (50%) and 600mg/kg (50%), and in male mice treated at dose 600mg/kg (85%). In addition, values of aspartate aminotransferase of male rats, and urea/BUN of female mice at both doses was also significantly reduced. No death was recorded, and the body weight changes remained normal as compared to the control. In gross observations, the kidneys and liver of treatment group appear normal in their texture, shape, sizeor color as compared to the control. Histopathological examination of liver and kidney were essentially normal. Conclusions: Findings in this study revealed that T. schimperi and T. serrulatus are relatively safe. However, mild focal mononuclear leukocytic infiltrations were seen in liver and kidney of mice treated at higher doses.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | T. schimperi, T. serrulatus, Herbal preparation, Toxicological assessment |
| Subjects: | Q Science > QM Human anatomy R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 27 Nov 2018 12:33 |
| Last Modified: | 27 Nov 2018 12:33 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7769 |
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