W/kidan, Samuel (2017) Evaluation of Antihyperglycemic Effect of Moringa Stenopetala Aquoeous Leaves Extract on Alloxan Induced Diabetic Rats. Masters thesis, Addis Ababa University.
![[img]](http://thesisbank.jhia.ac.ke/style/images/fileicons/application_pdf.png) |
PDF (Evaluation of Antihyperglycemic Effect of Moringa Stenopetala Aquoeous Leaves Extract on Alloxan Induced Diabetic Rats)
Samuel Weldekidan .pdf - Accepted Version Restricted to Repository staff only Download (975kB) | Request a copy |
Abstract
Background: Diabetes is a serious metabolic disorder with complications that results in significant morbidity and mortality. Current drugs used for diabetes therapy are not free from side effects and do not restore normal glucose homeostasis. Therefore, the purpose of this study is to evaluate antidiabetic, and pancreatic damage effect of Moringa stenopetala aqueous extract on alloxan induced diabetic rats. Methods:-The aqueous extract of Moringa stenopetala leaves 250 and 500mg/kg and Glibenclimide 5mg/kg/ body weight were administered to alloxan induced diabetic rats. All were administered orally using intragastric gavage for 28 days. Blood glucose level, Histopathological examination, liver and kidney functions, preliminary phytochemical screening tests were done. Result:-After IP administration of Alloxan monohydrate, the fasted blood glucose level rose in all experimentally induced diabetic groups. Administration of M. stenopetala leaves aqueous extract to diabetic rats showed remarkably reduction in blood glucose concentration, there were significant (P<0.05) difference on Day21 and 28 when compared with diabetic group. Moreover, treatment with both doses of M. Stenopetala extract to diabetic rats produced significant reduction in the blood glucose levels of rats when compared with diabetic group. Continuous oral administration for 28 days of the plant for diabetic rats led to a significant decrease in serum Urea, Creatinine, ALT, AST and ALP (P < 0.05) as compared with diabetic group who showed significant concentrations. This significant reduction was equivalent that occurred on application of glibenclamide. Levels of serum cholesterol remained unaltered in the experimental groups when compared with diabetic control. Histopathology of diabetic untreated rats revealed degeneration of pancreatic islet cells, which was due to alloxan used in this experiment. However, signs of regeneration of β-cells observed following consumption of Moringa stenopetala aqueous leaves extract as reported. Various phytochemical tests performed on the plant revealed the presence of different secondary metabolites. M. stenopetala leaves aqueous extract (250 and 500mg/kg) were improved the body weight of rats significantly from Day14-Day28 when compared with the Diabetic group. Conclusion:-The present study demonstrated that repeated oral administration of Moringa stenopetala aqueous leaf extracts (250mg/kg and 500mg/kg) for 28 days has shown beneficial effects on antihyperglycemia, body weight improvement restore biochemical changes of blood, improve alloxan pancreatic damage. Moringa stenopetala aqueous leaves extract have active ingredients responsible for antihyperglycemic effect. The phytochemical screening indicated the presence of alkaloids, phenols, flavonoids, tannins, terpenoids, steroids and saponins.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > QM Human anatomy R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 21 Jul 2018 10:24 |
| Last Modified: | 21 Jul 2018 10:24 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7920 |
Actions (login required)
 |
View Item |