Diagnostic Potential of Auramine O Staining for the Diagnosis of Leprosy

Girma, Selfu (2016) Diagnostic Potential of Auramine O Staining for the Diagnosis of Leprosy. Masters thesis, Addis Ababa University.

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Introduction: Mycobacterium leprae (M. leprae) is the causative agent of leprosy which is a chronic infectious disease. The disease mainly involves the skin and peripheral nerves. The 2015 WHO statistics showed that there were 213,899 new cases with the detection rate of 3.81 per 100,000 population globally in the year 2014. In Ethiopia, the trend of new case detection for the last ten consecutive years was in average 4200 per year. Implementation of more sensitive laboratory tests at medium and lower health facilities is crucial to reduce the disease transmission and leprosy associated disability. The most important aim this study is to avail Auramine O staining for diagnose of leprosy and improve the sensitivity of leprosy laboratory diagnosis on both SSS and skin biopsy section using Light Emitting Diode Florescent Microscope. Objective:To evaluate diagnostic potential of Auramine O staining for the diagnosis of leprosy using LED FM microscsope. Methodology: A cross sectional study was conducted on 142 leprosy and 28non-leprosy participants usingAuramine O, Zehil Neelson, Fite-Faraco, H&E and PCR tests on slit skin smear (SSS) and skin biopsy done at AHRI pathology laboratory.Tissue sections prepared from FFPE tissue was used for the stainings and alcohol fixed tissue was used to extract DNA for PCR. Sensititvity, specifity, positive (PPV) and negative predictive values(NPV), and Kappa values were calculated. Results:The Sensitivity and Specificity of ZN staining on SSS was 58.8% (95% CI:49.2% - 67.9%) and 100% (95% CI: 85.2% -100%); on the other hand Sensisivity and Specificity of Auramine O staining on SSS were 66.7% (95% CI:57.2% - 75.2%) and 100% (95% CI: 85.2% - 100), respectively. Fite-Faraco, Auramine O on tissue and H&E showedsensitivity of 78.1% , 79% and 86.8%, respectively. The sensititvity and specificity of PCRwas 89.5% (95% CI: 82.3% - 94.4%) and 89.5% (95% CI: 82.3% - 94.4%) respectively. Conclusion and recommendation:Auramine O staining on both SSS and tissue section was better compared with the previous routine diagnostic tests. The sensititvty of PCR was muchhigher and we give our emphasis to recommend Auramine O staining for the diagnosis of leprosy inlower health institutions and that of PCR diagnosis at a referral level.

Item Type: Thesis (Masters)
Subjects: Q Science > QR Microbiology
R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions: Africana
Depositing User: Emmanuel Ndorimana
Date Deposited: 23 Jul 2018 14:31
Last Modified: 23 Jul 2018 14:31
URI: http://thesisbank.jhia.ac.ke/id/eprint/7968

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