Eshetu, Seneshat (2017) Bacterial Profile and Antimicrobial Susceptibility Pattern of Blood Culture Isolates at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia. Masters thesis, Addis Ababa University.
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Abstract
Back ground: Bloodstream infections are major health problems that lead to morbidity and mortality of patients unless treated with appropriate antimicrobial treatment. Drug resistance of bacterial pathogens is challenging to alleviate sepsis. Blood culture is a gold standard technique which provides essential information for the diagnosis and proper medication. Objective: The Aim of study was to assess bacterial profile and antimicrobial susceptibility pattern of blood culture isolates at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia Method: A cross-sectional study was conducted from April 2016 to September 2016. Using convenient sampling technique a total of 422 blood sample were collected from study participant who were suspected for blood stream infection. Bacterial isolates were identified by colony morphology, gram staining reaction, biochemical tests such as catalase, coagulase, optochin, PYR, triple sugar iron agar (TSI), citrate utilization, urease, motility, Indole were used for bacterial identification and susceptibility to antibiotics was done using Kirby Bauer disc diffusion technique method according to CLSI guidelines in Microbiology Laboratory of Tikur Anbessa Specialized Hospital. The data were analyzed by using SPSS version 20. Result: About 66(15.6%) microbial were isolated. Out of this 64 (96.9%) were bacterial and 2(3.03%) were fungal (Candida albicans) isolates. The predominant bacterial isolates were S.aureus (22.72%) and K.pneumoniae (16.66%). Gram positive cocci were highly resistance to Penicillin (86.7%), followed by Ceftriaxone (46.7%), and 40% were MRSA.While clindamycin (80 %), erythromycin (73.3%), and Trimethoprim-Sulfamethoxazole (66.7%) were effective antibiotics. Most of gram negative rods were highly resistance to ampicillin (85.7%), amoxicillin clavulanic acid (77.14%), Ceftriaxone (75%), and Ceftazidimen (62.8%). Nevertheless amikacin (97.1%), meropenem (91.5%), and ciprofloxacin (77.1%) were sensitive antibiotic. Most of bacterial isolates (73.4%) were multidrug resistance to most frequently used antibiotics. Conclusion: Overall, there was a high prevalence of blood stream infection with high resistance rate to the most commonly used antibiotics. Hence, timely investigation of blood stream infection and regular surveillance of antimicrobial resistance pattern is important to reduce morbidity and mortality due to blood stream infection.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Blood stream infection, Bacterial profile, Antimicrobial sensitivity pattern |
| Subjects: | Q Science > QR Microbiology R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 29 Nov 2018 10:45 |
| Last Modified: | 29 Nov 2018 10:45 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7973 |
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