Bekele, Temesgen (2015) Invivo Anti-Malarial Evaluation of Leaf Extract of Vernonia Amygdalina Del. (Asteraceae) against Plasmodium Berghei. Masters thesis, Addis Ababa University.
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Abstract
Introduction: Malaria is one of the world’s most serious infectious diseases caused by Plasmodium parasites. An increased drug resistance to conventional anti-malarial, increasing resistance of mosquito vectors to insecticides, challenge of having effective vaccines and adverse effects of the existing anti-malarial drugs justifies the urgent need for more effective, tolerable and affordable anti-malarial drugs. Objective: In the attempt to search for new antimalarial drugs, the present study aimed to evaluate antimalarial activity and acute toxicity of crude extract of Vernonia amygdalina leaf extracts. Methods: The leaf of Vernonia amgdalina extract were prepared by cold maceration technique, four day suppressive, curative and prophylaxis test in mouse model was done to cheek the effect of the plant extracts against Plasmodium berghei in Swiss albino mice. Extracts were administered at doses of (200, 400 and 600) mg/kg body weight of mice. Data obtained from the experiment was analyzed using paired t-test and one way ANOVA. Results: The present study indicated that the extract did not exhibit any signs of acute toxicity up the dose of 2000mg/kg. The aqueous and hydrometanol extracts of V. amygdalina showed a parasitemia chemosuppression at the dose of 600 mg/kg with 68.8 and 69.2 % (p<0.0001) in 4-day suppressive test, 69.3 and 70.8% in curative model and 70.3 and 71.5% %(p<0.0001) in prophylactic model as compared to normal control, respectively, with significant (p<0.0001) effect on survival time compared to normal control. In Curatve test of the present study, the extract had shown prevention against Packed cell volume (PCV) and weight reduction significantly (p<0.05), as compared to normal control, in dose dependent manner. But the 4 days suppression and prophylactic model did not prevent reduction of PCV and weight fall. In all three models, the extract did not cause significant prevention of rectal temperature decrement. Conclusion: The extracts showed parasitemia suppression and a promising curative and prophylaxis activities in dose dependent manner.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Antimalarial activity, V. amygdalina, P. berghei, traditional medicine, invivo |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 26 Jul 2018 07:20 |
| Last Modified: | 26 Jul 2018 07:20 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/8167 |
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