Eticha, Temesgen (2016) Performance Evaluation of Care StartTM Malaria Pf/Pv Combo Test at Arbaminch General Hospital and Health Center, Gamo Gofa Zone, South Ethiopia. Masters thesis, Addis Ababa University.
![[img]](http://thesisbank.jhia.ac.ke/style/images/fileicons/application_pdf.png) |
PDF (Performance Evaluation of Care StartTM Malaria Pf/Pv Combo Test at Arbaminch General Hospital and Health Center, Gamo Gofa Zone, South Ethiopia)
Temesgen Eticha.pdf - Accepted Version Restricted to Repository staff only Download (812kB) | Request a copy |
Abstract
Background: Malaria remains endemic in 106 countries while parasite-based diagnosis increasing. Most suspected cases of malaria still not properly confirmed, resulting in over-use of anti-malarial drugs and poor disease monitoring. Malaria rapid diagnostic tests (RDTs) are alternative diagnostic methods which have enabled reliable biological diagnostic testing in all situations where previously only clinical diagnosis was available. The diagnostic accuracy of RDTs can vary significantly across different geographical regions. This variable quality of malaria RDTs, and consequently their diagnostic performance, have made it difficult for policy makers to determine which tests are the most suitable. Objective: The aim of the study was to evaluate the diagnostic performance of CareStartTM Malaria Pf/Pv Combo RDT by comparing with light microscope for malaria diagnosis in Arbaminch Hospital and Health center, Southern Ethiopia. Methods: A cross-sectional study design was conducted from October 1, 2015 up to October 15, 2016. A total of 160 patients were included in the study. Socio demographic data collection was done using structured questionnaire. Finger prick blood sample was obtained from those study subjects for Care Start™ Malaria Pf/Pv combo test, thin and thick film was prepared immediately for microscopic examination. Collected data from this study was entered using Epi Data version 3.1 and analyzed using SPSS version 20.0 and Microsoft Excel 2013. Sensitivity, specificity, PPV and NPV was determined in comparison with the gold standard Microscopy method. Result: In this study, it was found that Care start TM combo test had an overall sensitivity, specificity, PPV, and NPV of 97.44%, 93.67%, 93.83%, and 97.37% respectively, with Kappa value of 0.877 to detect the presence or absence of malaria. Sensitivity and specificity of the kit for p. falciparum detection was 63.27 % and 94.3% and for p. vivax detection was 86.96 % and 95.62 % respectively, which is below WHO standard. Agreement between Light microscopy and Care Start TM malaria RDT for specific identification of malaria species is moderate with kappa value of 0.568. It showed better performance at Arbaminch health center than Arbaminch Hospital with sensitivity, specificity, PPV, and NPV of 96.07%, 80%, 92.45% and 88.88% respectively. Conclusion and recommendations: The kit evaluated has good overall performance in detection of malaria cases which is almost comparable with the Gold standard microscopymethod. But, since it has still drawback in correctly identifying malaria species, further studies should be carried out to use the CareStartTM Malaria Pf/Pv Combo Test instead of microscopy for the diagnosis of malaria.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 26 Jul 2018 07:24 |
| Last Modified: | 26 Jul 2018 07:24 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/8172 |
Actions (login required)
 |
View Item |