Mengesha, Tewodros (2016) Hepatoprotective Effect of Silymarin on Fructose Induced Nonalcoholic Fatty Liver Disease in Male Albino Wistar Rats. Masters thesis, Addis Ababa University.
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PDF (Hepatoprotective Effect of Silymarin on Fructose Induced Nonalcoholic Fatty Liver Disease in Male Albino Wistar Rats)
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Abstract
Background: Nonalcoholic fatty liver disease is one of the most common causes of chronic liver disease in the Western world, and it‟s likely to parallel the increasing prevalence of type 2 diabetes, obesity, and other components of metabolic syndrome. There is also growing evidence in both animal models and human studies suggesting that high dietary intake of fructose is an important nutritional factor in the development of metabolic syndrome and its associated complications of NAFLD. However, an optimal treatment for NAFLD has not been established. Silymarin is gaining increasing recognition due to its beneficial effects in the control and prevention of alcoholic liver disease, acute and chronic viral hepatitis and toxin-induced liver diseases. Objective: The current study aim to investigate the hepatoprotective effect of silymarin on fructose induced nonalcoholic fatty liver disease in rats. Methodology: Thirty male Wistar rats were randomly divided into five groups: normal control group that consumed tap water; Silymarin control group that consumed tap water + silymarin (400 mg/kg/day), fructose control group that consumed 20% fructose solution; Treatment group that consumed 20% fructose solution + Silymarin (200 mg/kg/day) and treatment group that consumed 20% fructose solution + silymarin (400 mg/kg/day). The food and liquid intake, body weight, liver triglyceride, serum lipid profile, lipid peroxidation, antioxidant level and morphological as well as histopathological changes of liver were investigated. The data were analyzed using one way analysis of variance (ANOVA) followed by tukey multiple comparison test and statistical significance was determined at p ˂ 0.05. Result. This study showed that the fructose consumed model group had a significantly high values in the stage of steatosis grade, liver weight, hepatic triglyceride, serum triglyceride, total cholesterol, low density lipoprotein cholesterol, alanine amino transferase, aspartate aminotransferase and hepatic malondialdehyde concentration as compared to the normal control and contrariwise a significantly low values of reduced glutathione, plasma total antioxidant capacity and food consumption. The alteredparameters in the fructose model group had been partially ameliorated when treated with silymarin. Conclusion: The rats fed with fructose developed dyslipidemia, oxidative stress and mild steatosis in liver, which are the characteristics feature of nonalcoholic fatty liver disease. When treated with silymarin showed amelioration in oxidative stress, dyslipidemia and steatosis of liver in some extent but could not reach the normal control values.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Nonalcoholic Fatty Liver Disease, Silymarin; Lipid Peroxidation; Dyslipidemia; Total Antioxidant Status; Reduced Glutathione |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 02 Aug 2018 09:29 |
| Last Modified: | 02 Aug 2018 09:29 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/8239 |
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