Tesfaye, Tigist (2016) Investigation of the Chemopreventive and Hepatoprotective Potential of Terminalia Brownii Extract in the Wistar Rat Model of Colorectal Precancerous Lesions Induced and Promoted by DMH. Masters thesis, Addis Ababa University.
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Abstract
Background: Colorectal cancer has been one of the most deadly cancer in developed nations for a long time. Though the incidence of colorectal cancer has been low in developing nations, due to a rapid life style modification and adaptation of western lifestyles its incidence is increasing dramatically in recent years. Objective:The study investigates chemopreventive potential of Terminalia brownii stem extracts in a Wistar rat model of colorectal precancerous lesions induced and promoted by dimethylhydrazine (DMH). Method: Stem of Terminalia brownii were extracted using two kinds of solvent. i.e. water and 70% ethanol. The acute toxicity of the two types of extracts was checked in a male Wistar rats and the LD50 of the aqueous and 70% ethanol T. brownii were determined. Nine groups of Wistar rats each having a sample size of five rats were given IP injections of DMH (20mg/kg) weekly for five consecutive weeks while a control group received IP injection of normal saline. At the end of the fifth week each group of rats were treated by oral gavage with different concentrations of T. brownii extract. Three of the groups took aqueous extract of T. brownii at 250mg/kg, 500mg/kg and 750mg/kg dose daily for four weeks. The other three groups of rats took 70% ethanol extract of T. brownii at 250mg/kg, 500mg/kg and 750mg/kg dose daily for four weeks. One group of rats was given oral capecitabine at 30mg/kg/day for fourteen days. Another group of rats took capecitabine30mg/kg/day in combination with vitamin C 100mg/kg/day for fourteen days. The rats that took normal saline IP injection and one group of rats that took the DMH injection took the same amount of normal saline orally for four weeks. At the end of the experimental period blood was drawn from the heart and the rats were sacrificed for biochemical and histological analysis. Result: The findings of the study indicate that the incidence of Multiple Plaque Lesions (MPL) in DMH only treated group was 100% while the incidence of MLP was 0% in normal saline group. Groups which were treated with T. brownii showed a significant reduction in MLP incidence. Especially the 750mg/kg hydroethanolic T. brownii extract treated group showed 0% MLP incidence similar to the capecitabine and normal saline group. Also significant elevation of liver function tests were noted in capecitabine treated groups while lower liver function tests were noted in groups treated with vitamin C and T. brownii. Conclusion: This study showed that both the aqueous and hydroethanolic Terminalia brownii extract have chemopreventive and hepatoprotective potential. The aqueous extract showed a better hepatoprotective potential while the hydroethanolic extract showed a better chemopreventive potential.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Colorectal cancer, Terminalia brownii, DMH, Capecitabine |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology R Medicine > R Medicine (General) |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 27 Jul 2018 13:01 |
| Last Modified: | 27 Jul 2018 13:01 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/8291 |
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