Nega, Tihitna (2016) Immunological and Lipid Profile among Leprosy Patients at ALERT Centre, Addis Ababa Ethiopia. Masters thesis, Addis Ababa University.
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PDF (Immunological and Lipid Profile among Leprosy Patients at ALERT Centre, Addis Ababa Ethiopia)
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Abstract
Background: Leprosy is an old chronic infectious disease that continues to be an important public health problem in several developing countries.Host immunologic factors contribute to the clinical outcome in leprosy. This infection is also accompanied by several alterations in lipid metabolism and changes inthesynthesis of a number of acute phase proteins. Objective: To assess the immunological and lipid profile of leprosy patients across the disease spectrum Material and Methods: A crossectional study was conducted at ALERT center which included 30 leprosy patients and 21 healthy controls. An 8 ml of blood sample was collected from30 patients and 21 healthy controls and used for immunological and lipid profile assays. About 200µl of whole blood was stained with antibody conjugated flourochromes and data acquisition was done on FACSCanto II flowcytometer. Lipid profile and C reactive protein tests have been done using chemistry analyzer and qualitative test respectively. Mann-Whitney U test were used to analyze Memory T cells and lipid profile analysis. Result:The percentage of memory T cells (CD45RO+) expressed on CD3+ was significantly higher in PB patients than MB patients. Activated T cells (CD62L-) expressed on CD4+ were significantly higher in PB patients than in MB patients. MB patient’s CD3+ effector T cells were significantly higher than the healthy controls. PB patients showed significantly higher CD3+, CD4+ and CD8+ effector memory cells than MB patients. Regarding the lipid profile it showed that, healthy control serum total cholesterol level was significantly higher than MB patients. On the contrary serum HDL level was significantly higher in healthy control than MB patients. Conclusion: This study provides further evidence that there is a significant difference in lipid profile among leprosy spectrum. The percentage of both activated and memory T cells in MB patients are significantly lower than in PB patients and this confirms the unresponsiveness of T cells in MB patients regardless of the high bacterial load in these patients. Recommendation:We recommend that monitoring and regulate the lipid homeostasis of leprosy patients may be useful. We also recommend further study on host immune responses in leprosy diseases in which lipid metabolism and inflammation intersect.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > QR Microbiology R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 27 Jul 2018 09:52 |
| Last Modified: | 27 Jul 2018 09:52 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/8309 |
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