Tefera, Negusu (2009) Potential of Prosopis Juliflora for Bioethanol Production. Masters thesis, Addis Ababa University.
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Abstract
This study was aimed to determine the ethanol potential of prosopis juliflora pod by fermentation using saccharomyces cerevisiae yeast. The milled p.juliflora pod was hydrolysed by distilled water and by dilute sulfuric acid (0.2, 0.4, and 0.8 M) at temperature of 90°C for 15 and 30 minutes. The result showed that the maximum glucose (113.2 g/l) was obtained from hydrolysis of 100 g p. juliflora pod flour by 250 ml distilled water at 90 for 30 minutes followed by, 92.4 g/l, 68.1 g/l, and 56.1 g/l with 0.2, 0.4 and 0.8 M sulfuric acid respectively. This shows that treatment of Prosopis juliflora pod flour releases less glucose with increasing concentrations of sulfuric acid ranging from 21% with 0.2M to that of 50% reduction with 0.8M acid treated hydrolysatecompared to the glucose concentration of water treated pod flour. NMR analysis of the fermented hydrolysate gives maximum ethanol concentration of (5% w/v) with water and, 4%, 3 % and 2.5% of ethanol with 0.2, 0.4 M and 0.8 M sulfuric acid hydrolysis at 90oC for 30 minutes respectively. Treatment with water is an effective way that result the maximum yield and treatment with 0.8M acid is the least effective. 15 minute hydrolysis resulted lower ethanol 4%, 2.7%, 2%, and 1.6% with water, 0.2, 0.4 and 0.8M sulfuric acid respectively. The optimum fermentation time was 24 hour which gives maximum concentration of ethanol (5%w/v) with water and the concentration decreases slightly after 24 hours fermentation.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology Q Science > QK Botany |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 24 May 2018 12:28 |
| Last Modified: | 24 May 2018 12:28 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/4095 |
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