Woldu, Anteneh Yihune (2011) InVitro Study of the Effects of Coffee and Thyme on Isolated Airway Smooth Muscle Tissue of Guinea pigs. Masters thesis, Addis Ababa University.
![[img]](http://thesisbank.jhia.ac.ke/style/images/fileicons/application_pdf.png) |
PDF (InVitro Study of the Effects of Coffee and Thyme on Isolated Airway Smooth Muscle Tissue of Guinea pigs)
Anteneh, Yihune.pdf - Accepted Version Restricted to Repository staff only Download (3MB) | Request a copy |
Abstract
BACKGROUND: Coffea arabica and Thymus schimperi are plants claimed in traditional medicine to possess numerous beneficial/healing effects including therapeutics in asthma due to airway smooth muscle relaxant properties. The objective of this study was to investigate the bronchodilator effects of these plants crude extracts on guinea pig trachea smooth muscles. METHODS: Isolated guinea-pig trachea rings were used to study the effects of hydroalcholic and n-hexane crude extracts of the beans of Coffea arabica and the leaves of Thymus schimperi in vitro in the presence of acetylcholine by using single dosage method of extract administration. Salbutamol was used as a standard drug. RESULTS: Investigation of the effects hydroalcholic (methanol 80%) and n-hexane crude extract of Coffea arabica L. and Thymus schimperi R. on an isolated trachea smooth muscle tissue in the presence of Ach (in vitro) yielded an overall contractile response. The contractile responses of the plant crude extracts in the absence of Ach were clearly evident on the isolated tissue to variable degrees depending on type of plant, type of plant extract and amount of dosage administered to the tissue. The tissue contractile response to Ach (160ng) alone, which peaked at 1.75mm (mean peak point) from baseline on the polygraph chart, was used as a positive control for contraction. In the presence of the plant extracts (both plants and types of extracts), the contractile response of the GPT when Ach was added had an amplified (additive) contractile effect except for the 50µg dose group of n- hexane crude extract of Coffea arabica, which showed a relaxant effect of 0.22mm in the presence of Ach and was not of statistical significance. Statistical analysis indicated that the hydroalcholic crude extracts of Coffea arabica and Thymus schimperi showed significant differences in tissue contraction between the 50µg dose and 100 µg, 200 µg, 400 µg group and between the 50µg dose the two dose groups of 150 µg and 200 µg respectively (Pvalue� 0.05). CONCLUSION: The hydroalcholic (Methanol 80%) crude extracts of both Coffea arabica L. and Thymus schimperi R. showed a contractile response on the guinea pig trachea rings in vitro which was statistically significant between the dose groups in the presence of Ach. The results yielded are not in full agreement to the traditional claim of a relaxant effect on the airways.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Coffee, Thyme, Smooth Muscle, Guinea Pig Trachea Rings |
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QK Botany Q Science > QL Zoology |
| Divisions: | Africana |
| Depositing User: | Selom Ghislain |
| Date Deposited: | 16 Aug 2018 08:35 |
| Last Modified: | 16 Aug 2018 08:35 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/4812 |
Actions (login required)
 |
View Item |