Evaluation of Wound Healing and Anti-Inflammatory Activity of 80% Methanolic Extract of Solanum incanum Linnaeus (Solanaceae) Leaves in Mice

Doyo, Debesa (2016) Evaluation of Wound Healing and Anti-Inflammatory Activity of 80% Methanolic Extract of Solanum incanum Linnaeus (Solanaceae) Leaves in Mice. Masters thesis, Addis Ababa University.

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Abstract

Solanum incanum Linnaeus or Sodom/bitter apple (English), Embouy(Amharic), Hiddi(Afan Oromo) is a perennial, wild shrub like herb that grows up to 1.8 m in height that belongs to Solanaceae family which grows in many regions of Africa. In Africa, including Ethiopia the herb is used as a folklore remedy for different ailment such as skin problem like wound and inflammations. However, to date, no scientific report could be found in the literature concerning the in-vivo wound healing and anti-inflammatory activity. The purpose of this study is therefore to evaluate in- vivo wound healing and anti-inflammatory activity of 80% methanol extract of S. incanum L. leaves in mice. Extracts were studied for wound healing activity topically by incorporating in simple ointment base B.P. in concentration of 5% (w/w) and 10% (w/w). The animals were randomly divided into four groups in wound healing models and five groups (each containing six mice) for anti-inflammatory activity test. Simple ointment base (negative control) and nitrofurazone 0.2%(w/w)ointment was used as standard for activity comparison. For the study of in- vivo anti-inflammatory activity, the 80% methanolic extract was dissolved in 2% Tween 80. Two models were used for wound healing activity in mice viz. excision and incision. Carrageenan induced hind paw edema model was used for anti-inflammatory study. Parameters such as wound contraction, and period of epithelialization were studied in case of the excision wound model, while incision wound model was evaluated by determining tensile strength. Leaves extract of S. incanum was given orally at dose of 100, 200 and 400 mg/kg in carrageenan induced hind paw oedema model and oedema was evaluated by determining mean increase in paw volume and percentage edema inhibition. Indomethacin 10mg/kg was used as reference standard for the activity comparison. Treatment of wound with ointment containing 5%(w/w) and 10% (w/w) 80% methanol extract exhibited significant(p<0.05) increase in wound contraction rate, shorter epithelialization time and higher skin breaking strength in the two experimental model as compared to control. S. incanum L. extract also shows dose-dependent significant reductions (p < 0.05) of inflammation as compared to control. These results collectively demonstrate that the 80% methanol extract of S. incanum L. possesses wound healing and anti-inflammatory properties, and this justifies the use of the leaves of S. incanum L. for wound and inflammations as claimed in the folklore literature.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Solanum incanum L., wound healing activity, excision wound model, incision wound model, tensile strength, Anti-inflammatory, carrageenan
Subjects:	Q Science > Q Science (General) R Medicine > RM Therapeutics. Pharmacology
Divisions:	Africana
Depositing User:	Vincent Mpoza
Date Deposited:	17 Oct 2018 07:49
Last Modified:	17 Oct 2018 07:49
URI:	http://thesisbank.jhia.ac.ke/id/eprint/6861

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