Samuel, Mesfin (2016) Comparision of Hemocue301+ with Sysmex-KX21N and Celldyn1800 Automated Hematology Analyzers for Measurement of Hemoglobin Levels in Patients at St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia, 2015. Masters thesis, Addis Ababa University.
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Abstract
Background:Hemoglobin concentration is measured routinely using automated hematology analyzers. Even if these automated hematology analyzers are very accurate and reliable, they are expensive and transport of the samples to the laboratory delays the process which may delay treatment. Point of care hemoglobin analyzer like Hemocue301+ has potential to solve such problems. Although method comparison experiment has to be conducted when new instrument are installed, this practice is not common in our country. Objective:To compare hemoglobin determined by Hemocue301+, with Sysmex-kx21N and Cell-Dyn1800 methods using patients’ blood sample at St Paul’s Specialized Hospital Millennium Medical College in Addis Ababa, Ethiopia. Methods:A facility based cross sectional study with convenient sampling method was conducted at St. Paul’s specialized teaching hospital starting from May 15 to June 2 2015. A total of 117specimens for agreement study and 3 specimens for precision study were analyzed on Hemocue301+, Sysmex-Kx21N, and cell-Dyn1800 hematology analyzers to compare their Hemoglobin results. This study includes patients of all age group. One sample t-test and bland and Altman plot with limit of agreement were used to make inference. Data analyses were performed using SPSS version 20 Software. Result:The precision experiment showed within run normal range CV% for Hemocue301+, SysmexKx21N and Cell-Dyn1800 (1.7%, 1.3%, 1.8%, respectively). The one sample t-test showed a non-significant p-value of 0.10 for Hemocue301+ and Sysmex-Kx21N and the limit of agreement from Bland and Altman plot for 95% was (-1.07, 1.25)g/dl with only 1.7% of the cases were out of the limit of agreement. For Hemocue301+ and Cell-Dyn1800, a non-significant p- value of 0.46 and the Bland and Altman plot limit of agreement for 95% was (-1.22, 1.31)g/dl with only 1.7%of the case were out of the limit of agreement.Hemocue301+was agreed with SysmexKx21N and Cell-Dyn1800 within 1 g/dl which was considered as clinically important limit for Hemoglobin measurement. Conclusion: The Hemocue301+ maybe used interchangeably with Sysmex-Kx21N and CellDyn1800 automated Hematology analyzers in St Paul’s Specialized Hospital Millennium Medical College Hematology laboratory.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Africana |
| Depositing User: | Emmanuel Ndorimana |
| Date Deposited: | 12 Jul 2018 10:36 |
| Last Modified: | 12 Jul 2018 10:36 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7353 |
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