Eshetu, Amelework (2007) Diuretic Efficacy Evaluation of the Crude extracts & Fractions of Thymus Serrulatus. Masters thesis, Addis Ababa University.
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Abstract
Thymus serrulatus (in Amharic called ‘tosign’) is used traditionally for different ailments like hypertension, abdominal cramps, parasitic infections of animals, as food additives and spice in flavoring tea. It is also used as anthelmintic, antiseptic, antispasmodic, carminative, deodorant, diaphoretic, disinfectant, expectorant, sedative and tonic. There is also a claim that T. serrulatus has diuretic activity and based on this claim. The aim of the present study was therefore to evaluate the diuretic activity of the crude hydroalcholic, aqueous and their fractions and to determine LD50 acute toxicity test of T. serrulatus on mice. The diuretic activity of the extracts was assessed by administering different dose levels orally. Vehicle and hydrochlorothiazide treated mice were used as negative and positive controls respectively. Both the crude methanol and aqueous extracts significantly increased the urinary output and urinary excretion of Na+ and K+ at all dose levels (P<0.05). The n-butanol fractions of both aqueous and methanol extracts significantly increased the urinary output as well as urinary electrolytes; the effect was comparable to that of the standard drug, hydrochlorothiazide. The dichloromethane, chloroform and aqueous fractions of the both extracts had no effect on the urine output and electrolyte excretion at all dose levels (P>0.05). Acute toxicity test, at a limit dose of 5g/kg of n-butanol fraction (the most active fraction) of the methanol and aqueous extract showed no behavioral change or sign of toxicity in the first four hours and no mortality was recorded after 24h. The experimental finding suggested that T. serrulatus has diuretic effect supporting the traditional claim of its use as a diuretic agent.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Africana |
| Depositing User: | Vincent Mpoza |
| Date Deposited: | 21 Nov 2018 08:24 |
| Last Modified: | 21 Nov 2018 08:24 |
| URI: | http://thesisbank.jhia.ac.ke/id/eprint/7554 |
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